Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/18959
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dc.contributor.authorThiab, Noor Riyadhen
dc.contributor.authorKing, Nicolaen
dc.contributor.authorMcMillan, Maryen
dc.contributor.authorAlghamdi, Othman Ahmed Sen
dc.contributor.authorJones, Graham Len
dc.date.accessioned2016-05-06T16:10:00Z-
dc.date.issued2016-
dc.identifier.citationAIMS Molecular Science, 3(2), p. 125-137en
dc.identifier.issn2372-0301en
dc.identifier.issn2372-028Xen
dc.identifier.urihttps://hdl.handle.net/1959.11/18959-
dc.description.abstractSmall molecular weight oxygen free radical species (ROS) involved in oxidative stress can cause damage to cellular macromolecules including proteins, DNA and lipids. One of the most important enzymes involved in ROS detoxification is glutathione peroxidase (GPx). Here we study the age-related expression of GPx isoenzymes in various parts of the rat kidney with and without exposure to external oxidative stress. These results are correlated to the age dependent changes in the expression of the chaperone, 'Hsp-70'. Protein and mRNA expression of GPx1 and GPx4 was studied in different regions of the kidney in ageing rats in the presence and absence of the external stressor 0.2 mM H₂O₂. Protein levels were examined by Western blot analysis following detection with appropriate antibodies and mRNA levels were analysed by quantitative reverse transcription polymerase chain reaction (qRT-PCR) using appropriate primer sequences. mRNA expression for the chaperone 'Hsp70' was investigated in parallel. After reaching a peak at maturity (12 weeks), GPx1 protein and mRNA levels decreased with age under both control and stress conditions, and were higher in the cortex than in the outer and inner medulla. GPx4 protein and mRNA levels showed few comparable age-related changes. By contrast with the observed age-related decrease in GPx1 expression, chaperone 'Hsp-70' mRNA expression greatly increased with age. These findings suggest that the age-related decline in GPx1 expression in the cortex may be partly offset by a reciprocal change in 'Hsp-70' expression. These results are consistent with the oxidative stress theory of ageing.en
dc.languageenen
dc.publisherAmerican Institute of Mathematical Sciencesen
dc.relation.ispartofAIMS Molecular Scienceen
dc.titleAge-related protein and mRNA expression of glutathione peroxidases (GPx) and Hsp-70 in different regions of rat kidney with and without stressoren
dc.typeJournal Articleen
dc.identifier.doi10.3934/molsci.2016.2.125en
dcterms.accessRightsGolden
dc.subject.keywordsMedical and Health Sciencesen
dc.subject.keywordsBiochemistry and Cell Biologyen
dc.subject.keywordsEnzymesen
local.contributor.firstnameNoor Riyadhen
local.contributor.firstnameNicolaen
local.contributor.firstnameMaryen
local.contributor.firstnameOthman Ahmed Sen
local.contributor.firstnameGraham Len
local.subject.for2008060199 Biochemistry and Cell Biology not elsewhere classifieden
local.subject.for2008119999 Medical and Health Sciences not elsewhere classifieden
local.subject.for2008060107 Enzymesen
local.subject.seo2008920119 Urogenital System and Disordersen
local.subject.seo2008929999 Health not elsewhere classifieden
local.profile.schoolScience and Technologyen
local.profile.schoolSchool of Science and Technologyen
local.profile.schoolHuman Biology and Physiologyen
local.profile.schoolSchool of Science and Technologyen
local.profile.emailnthiab@une.edu.auen
local.profile.emailNicola.King@plymouth.ac.uken
local.profile.emailmrookle2@une.edu.auen
local.profile.emailoalghamd@une.edu.auen
local.profile.emailgjones2@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20160427-115936en
local.publisher.placeUnited States of Americaen
local.format.startpage125en
local.format.endpage137en
local.peerreviewedYesen
local.identifier.volume3en
local.identifier.issue2en
local.access.fulltextYesen
local.contributor.lastnameThiaben
local.contributor.lastnameKingen
local.contributor.lastnameMcMillanen
local.contributor.lastnameAlghamdien
local.contributor.lastnameJonesen
dc.identifier.staffune-id:nthiaben
dc.identifier.staffune-id:mrookle2en
dc.identifier.staffune-id:oalghamden
dc.identifier.staffune-id:gjones2en
local.profile.orcid0000-0002-2336-3985en
local.profile.orcid0000-0002-6435-1542en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:19158en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleAge-related protein and mRNA expression of glutathione peroxidases (GPx) and Hsp-70 in different regions of rat kidney with and without stressoren
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.search.authorThiab, Noor Riyadhen
local.search.authorKing, Nicolaen
local.search.authorMcMillan, Maryen
local.search.authorAlghamdi, Othman Ahmed Sen
local.search.authorJones, Graham Len
local.uneassociationUnknownen
local.year.published2016en
local.subject.for2020310106 Enzymesen
local.subject.for2020320599 Medical biochemistry and metabolomics not elsewhere classifieden
local.subject.for2020310199 Biochemistry and cell biology not elsewhere classifieden
local.subject.seo2020280112 Expanding knowledge in the health sciencesen
local.subject.seo2020280103 Expanding knowledge in the biomedical and clinical sciencesen
local.subject.seo2020209999 Other health not elsewhere classifieden
local.codeupdate.date2022-03-07T08:13:25.132en
local.codeupdate.epersonrtobler@une.edu.auen
local.codeupdate.finalisedtrueen
local.original.for2020undefineden
local.original.for2020310106 Enzymesen
local.original.for2020undefineden
local.original.seo2020undefineden
local.original.seo2020undefineden
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