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https://hdl.handle.net/1959.11/18780
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DC Field | Value | Language |
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dc.contributor.author | Denham, Joshua | en |
dc.contributor.author | O'Brien, Brendan J | en |
dc.contributor.author | Marques, Francine Z | en |
dc.contributor.author | Charchar, Fadi J | en |
dc.date.accessioned | 2016-03-31T15:32:00Z | - |
dc.date.issued | 2015 | - |
dc.identifier.citation | Journal of Applied Physiology, 118(4), p. 475-488 | en |
dc.identifier.issn | 1522-1601 | en |
dc.identifier.issn | 8750-7587 | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/18780 | - |
dc.description.abstract | Physical exercise has proven cardiovascular benefits, yet there is no clear understanding of the related molecular mechanisms leading to this. Here we determined the beneficial epigenetic effects of exercise after sprint interval training, a form of exercise known to improve cardiometabolic health. We quantified genome-wide leukocyte DNA methylation of 12 healthy young (18-24 yr) men before and after 4 wk (thrice weekly) of sprint interval training using the 450K BeadChip (Illumina) and validated gene expression changes in an extra seven subjects. Exercise increased subjects' cardiorespiratory fitness and maximal running performance, and decreased low-density lipoprotein cholesterol concentration in conjunction with genome-wide DNA methylation changes. Notably, many CpG island and gene promoter regions were demethylated after exercise, indicating increased genome-wide transcriptional changes. Among genes with DNA methylation changes, epidermal growth factor (EGF), a ligand of the epidermal growth factor receptor known to be involved in cardiovascular disease, was demethylated and showed decreased mRNA expression. Additionally, we found that in microRNAs miR-21 and miR-210, gene DNA methylation was altered by exercise causing a cascade effect on the expression of the mature microRNA involved in cardiovascular function. Our findings demonstrate that exercise alters DNA methylation in circulating blood cells in microRNA and protein-coding genes associated with cardiovascular physiology. | en |
dc.language | en | en |
dc.publisher | American Physiological Society | en |
dc.relation.ispartof | Journal of Applied Physiology | en |
dc.title | Changes in the leukocyte methylome and its effect on cardiovascular-related genes after exercise | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1152/japplphysiol.00878.2014 | en |
dcterms.accessRights | Gold | en |
dc.subject.keywords | Exercise Physiology | en |
dc.subject.keywords | Gene Expression (incl. Microarray and other genome-wide approaches) | en |
dc.subject.keywords | Epigenetics (incl. Genome Methylation and Epigenomics) | en |
local.contributor.firstname | Joshua | en |
local.contributor.firstname | Brendan J | en |
local.contributor.firstname | Francine Z | en |
local.contributor.firstname | Fadi J | en |
local.subject.for2008 | 060404 Epigenetics (incl. Genome Methylation and Epigenomics) | en |
local.subject.for2008 | 110602 Exercise Physiology | en |
local.subject.for2008 | 060405 Gene Expression (incl. Microarray and other genome-wide approaches) | en |
local.subject.seo2008 | 970106 Expanding Knowledge in the Biological Sciences | en |
local.profile.school | School of Science and Technology | en |
local.profile.email | jdenham2@une.edu.au | en |
local.output.category | C1 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.identifier.epublicationsrecord | une-20160321-081013 | en |
local.publisher.place | United States of America | en |
local.format.startpage | 475 | en |
local.format.endpage | 488 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 118 | en |
local.identifier.issue | 4 | en |
local.access.fulltext | Yes | en |
local.contributor.lastname | Denham | en |
local.contributor.lastname | O'Brien | en |
local.contributor.lastname | Marques | en |
local.contributor.lastname | Charchar | en |
dc.identifier.staff | une-id:jdenham2 | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:18981 | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Changes in the leukocyte methylome and its effect on cardiovascular-related genes after exercise | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.search.author | Denham, Joshua | en |
local.search.author | O'Brien, Brendan J | en |
local.search.author | Marques, Francine Z | en |
local.search.author | Charchar, Fadi J | en |
local.uneassociation | Unknown | en |
local.year.published | 2015 | en |
local.subject.for2020 | 310504 Epigenetics (incl. genome methylation and epigenomics) | en |
local.subject.for2020 | 420702 Exercise physiology | en |
local.subject.for2020 | 310505 Gene expression (incl. microarray and other genome-wide approaches) | en |
local.subject.seo2020 | 280102 Expanding knowledge in the biological sciences | en |
Appears in Collections: | Journal Article |
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