Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/1847
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dc.contributor.authorRenz, Katrinen
dc.contributor.authorWalkden-Brown, Steveen
dc.contributor.authorIslam, A Fakhrulen
dc.contributor.authorCheetham, Brian Fredericken
dc.date.accessioned2009-06-16T16:17:00Z
dc.date.created2008en
dc.date.issued2008en
dc.identifier.urihttps://hdl.handle.net/1959.11/1847en
dc.description.abstractChapter 1 reviews the literature on Marek's disease relevant to the work carried out in this thesis. Methods for TaqMan® quantitative real-time PCR (qPCR) assays to detect the three serotypes of Marek's disease virus (MDV) are already available, and absolute quantification has been developed for MDV serotype 1 and serotype 3. The development of a method for absolute quantification of Marek's disease virus serotype 2 (MDV2) is described in Chapter 2. Using plasmid DNA, the lower detection limit of the MDV2 assay was determined to be 10 copies of the viral genome. Three independent assay runs showed highly reproducible Ct values and calculated copy numbers, with mean intra- and interassay coefficients of variation of less than 3 % for Ct and 21.5 % for calculated copy number. Absolute quantification of MDV2 was performed successfully on dust samples collected from poultry farms across Australia, material from infectious spleens and feather tips from chickens vaccinated with an avirulent strain of MDV2. Thus, it is now possible to use qPCR assays for absolute quantification of all three serotypes of MDV in a sample. The sequencing of the 'meq' gene of 5 Australian isolatres of MDV1 isolated between 1992 and 2004 which had been pathotyped in an experiment using unvaccinated and HVT-vaccinated specific pathogen free (SPF) chickens is reported in Chapter 3. The sequencing results were compared with a range of MDV1 meq sequences published in GenBank® and associations with virulence examined.en
dc.languageenen
dc.title'In vitro' and 'in vivo' characterization of selected Australian isolates of Marek's disease virusen
dc.typeThesis Doctoralen
dcterms.accessRightsUNEgreenen
dc.subject.keywordsAnimal Protection (Pests and Pathogens)en
dc.subject.keywordsMicrobiologyen
local.contributor.firstnameKatrinen
local.contributor.firstnameSteveen
local.contributor.firstnameA Fakhrulen
local.contributor.firstnameBrian Fredericken
local.subject.for2008070205 Animal Protection (Pests and Pathogens)en
local.subject.for2008060599 Microbiology not elsewhere classifieden
local.subject.seo630106 Poultryen
dcterms.RightsStatementCopyright 2008 - Katrin Renzen
dc.date.conferred2008en
local.thesis.degreelevelDoctoralen
local.thesis.degreenameDoctor of Philosophyen
local.contributor.grantorUniversity of New Englanden
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.emailkrenz@une.edu.auen
local.profile.emailswalkden@une.edu.auen
local.profile.emailbcheetha@une.edu.auen
local.output.categoryT2en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune_thesis-20090523-080950en
local.access.fulltextYesen
local.contributor.lastnameRenzen
local.contributor.lastnameWalkden-Brownen
local.contributor.lastnameIslamen
local.contributor.lastnameCheethamen
dc.identifier.staffune-id:krenzen
dc.identifier.staffune-id:swalkdenen
dc.identifier.staffune-id:fislam2en
dc.identifier.staffune-id:bcheethaen
local.profile.orcid0000-0002-0638-5533en
local.profile.roleauthoren
local.profile.rolesupervisoren
local.profile.rolesupervisoren
local.profile.rolesupervisoren
local.identifier.unepublicationidune:1909en
local.identifier.handlehttps://hdl.handle.net/1959.11/1847en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitle'In vitro' and 'in vivo' characterization of selected Australian isolates of Marek's disease virusen
local.output.categorydescriptionT2 Thesis - Doctorate by Researchen
local.description.statisticsepubsVisitors: 1526<br />Views: 365<br />Downloads: 1241en
local.thesis.borndigitalyesen
Appears in Collections:School of Environmental and Rural Science
Thesis Doctoral
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