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Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/16430
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dc.contributor.authorHou, Xingshengen
dc.contributor.authorMcMillan, Maryen
dc.contributor.authorCoumans-Moens, Joelleen
dc.contributor.authorPoljak, Anneen
dc.contributor.authorRaftery, Marken
dc.contributor.authorPereg, Lilyen
dc.date.accessioned2015-01-08T16:23:00Z-
dc.date.issued2014-
dc.identifier.citationPLoS One, 9(12), p. 1-25en
dc.identifier.issn1932-6203en
dc.identifier.urihttps://hdl.handle.net/1959.11/16430-
dc.description.abstractFlcA is a response regulator controlling flocculation and the morphological transformation of 'Azospirillum' cells from vegetative to cyst-like forms. To understand the cellular responses of 'Azospirillum' to conditions that cause morphological transformation, proteins differentially expressed under flocculation conditions in 'A. brasilense' Sp7 and its flcA knockout mutant were investigated. Comparison of 2-DE protein profiles of wild-type (Sp7) and a flcA deletion mutant (Sp7-flcAΔ) revealed a total of 33 differentially expressed 2-DE gel spots, with 22 of these spots confidently separated to allow protein identification. Analysis of these spots by liquid chromatography-tandem mass spectrometry (LC-MS/MS) and MASCOT database searching identified 48 proteins (≥10% emPAI in each spot). The functional characteristics of these proteins included carbon metabolism (beta-ketothiolase and citrate synthase), nitrogen metabolism (Glutamine synthetase and nitric oxide synthase), stress tolerance (superoxide dismutase, Alkyl hydroperoxidase and ATP-dependent Clp protease proteolytic subunit) and morphological transformation (transducer coupling protein). The observed differences between Sp7 wild-type and flcA⁻ strains enhance our understanding of the morphological transformation process and help to explain previous phenotypical observations. This work is a step forward in connecting the Azospirillum phenome and genome.en
dc.languageenen
dc.publisherPublic Library of Scienceen
dc.relation.ispartofPLoS Oneen
dc.titleCellular Responses during Morphological Transformation in 'Azospirillum brasilense' and Its flcA Knockout Mutanten
dc.typeJournal Articleen
dc.identifier.doi10.1371/journal.pone.0114435en
dcterms.accessRightsGolden
dc.subject.keywordsBacteriologyen
dc.subject.keywordsMicrobial Geneticsen
dc.subject.keywordsMicrobial Ecologyen
local.contributor.firstnameXingshengen
local.contributor.firstnameMaryen
local.contributor.firstnameJoelleen
local.contributor.firstnameAnneen
local.contributor.firstnameMarken
local.contributor.firstnameLilyen
local.subject.for2008060504 Microbial Ecologyen
local.subject.for2008060501 Bacteriologyen
local.subject.for2008060503 Microbial Geneticsen
local.subject.seo2008970106 Expanding Knowledge in the Biological Sciencesen
local.profile.schoolSchool of Science and Technologyen
local.profile.schoolSchool of Rural Medicineen
local.profile.schoolSchool of Science and Technologyen
local.profile.emailmrookle2@une.edu.auen
local.profile.emailjmoensco@une.edu.auen
local.profile.emaillperegge@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20141218-152715en
local.publisher.placeUnited States of Americaen
local.identifier.runningnumbere114435en
local.format.startpage1en
local.format.endpage25en
local.identifier.scopusid84918561780en
local.peerreviewedYesen
local.identifier.volume9en
local.identifier.issue12en
local.access.fulltextYesen
local.contributor.lastnameHouen
local.contributor.lastnameMcMillanen
local.contributor.lastnameCoumans-Moensen
local.contributor.lastnamePoljaken
local.contributor.lastnameRafteryen
local.contributor.lastnamePeregen
dc.identifier.staffune-id:xhouen
dc.identifier.staffune-id:mrookle2en
dc.identifier.staffune-id:jmoenscoen
dc.identifier.staffune-id:lpereggeen
local.profile.orcid0000-0002-2336-3985en
local.profile.orcid0000-0001-6642-5202en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:16667en
local.identifier.handlehttps://hdl.handle.net/1959.11/16430en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleCellular Responses during Morphological Transformation in 'Azospirillum brasilense' and Its flcA Knockout Mutanten
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.search.authorHou, Xingshengen
local.search.authorMcMillan, Maryen
local.search.authorCoumans-Moens, Joelleen
local.search.authorPoljak, Anneen
local.search.authorRaftery, Marken
local.search.authorPereg, Lilyen
local.uneassociationUnknownen
local.identifier.wosid000346375400031en
local.year.published2014en
local.subject.for2020310703 Microbial ecologyen
local.subject.for2020310701 Bacteriologyen
local.subject.for2020310704 Microbial geneticsen
local.subject.seo2020280102 Expanding knowledge in the biological sciencesen
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