Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/15092
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dc.contributor.authorAlghamdi, Othman Ahmed Sen
dc.contributor.authorMoens, Pierreen
dc.contributor.authorJones, Graham Len
dc.contributor.authorKing, Nicolaen
local.source.editorEditor(s): Guoyao Wuen
dc.date.accessioned2014-05-16T11:08:00Z
dc.date.issued2013en
dc.identifier.citationAmino Acids, 45(3), p. 588-588en
dc.identifier.issn1438-2199en
dc.identifier.issn0939-4451en
dc.identifier.urihttps://hdl.handle.net/1959.11/15092en
dc.description.abstractPEPT1 and PEPT2 are H+-dependent cotransporters found in the kidney. They are important for the reabsorption of small peptides and are also capable of transporting a broad range of peptidomimetic drugs. Due to the electrogenic nature of these carriers, transport of individual substrates should be accompanied by a positive change in the membrane potential difference (pd). The aim of this study was to investigate a novel use for the potential sensitive fluorescent dye 3,30-dipropylthiacarbocyanine iodide [DiS-C₃-(3)] as a biosensor for dipeptide transport across the renal proximal tubule apical membrane. Brush border (BBMV) and outer medulla (OMMV) membrane vesicles were isolated from male Wistar rats using standard techniques. Fluorescence changes were measured when extracellular media at pH 6.6 containing 0-3 mM L-glycyl-L-glutamine (gly-gln) was added to a cuvette containing vesicles pre-equilibrated at pH 7.4 and 7.5 μM DiS-C₃-(3). Data presented are means ± SE of n = 6. A positive change in fluorescence occurred upon gly-gln addition reflecting the expected positive change in membrane pd.en
dc.languageenen
dc.publisherSpringer Wienen
dc.relation.ispartofAmino Acidsen
dc.titleA new use of the biosensor Di-C₃-(3) to measure dipeptide transport across the apical membrane of the renal proximal tubuleen
dc.typeConference Publicationen
dc.relation.conference13th International Congress on Amino Acids, Peptides and Proteins, Galveston, United States of America, 5th - 7th October, 2013en
dc.identifier.doi10.1007/s00726-013-1540-yen
dc.subject.keywordsBiochemistry and Cell Biologyen
local.contributor.firstnameOthman Ahmed Sen
local.contributor.firstnamePierreen
local.contributor.firstnameGraham Len
local.contributor.firstnameNicolaen
local.subject.for2008060199 Biochemistry and Cell Biology not elsewhere classifieden
local.subject.seo2008920119 Urogenital System and Disordersen
local.profile.schoolHuman Biology and Physiologyen
local.profile.schoolSchool of Science and Technologyen
local.profile.schoolSchool of Science and Technologyen
local.profile.schoolSchool of Science and Technologyen
local.profile.emailoalghamd@myune.edu.auen
local.profile.emailpmoens@une.edu.auen
local.profile.emailgjones2@une.edu.auen
local.profile.emailnking20@une.edu.auen
local.output.categoryE3en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20140513-134326en
local.publisher.placeAustriaen
local.format.startpage588en
local.format.endpage588en
local.identifier.volume45en
local.identifier.issue3en
local.contributor.lastnameAlghamdien
local.contributor.lastnameMoensen
local.contributor.lastnameJonesen
local.contributor.lastnameKingen
dc.identifier.staffune-id:oalghamden
dc.identifier.staffune-id:pmoensen
dc.identifier.staffune-id:gjones2en
dc.identifier.staffune-id:nking20en
local.profile.orcid0000-0003-3121-5306en
local.profile.orcid0000-0002-6435-1542en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:15307en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleA new use of the biosensor Di-C₃-(3) to measure dipeptide transport across the apical membrane of the renal proximal tubuleen
local.output.categorydescriptionE3 Extract of Scholarly Conference Publicationen
local.description.statisticsepubsVisitors: 312<br />Views: 308<br />Downloads: 0en
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