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https://hdl.handle.net/1959.11/13944
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DC Field | Value | Language |
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dc.contributor.author | Shavlakadze, T | en |
dc.contributor.author | Anwari, T | en |
dc.contributor.author | Soffe, Z | en |
dc.contributor.author | Cozens, G | en |
dc.contributor.author | Mark, P J | en |
dc.contributor.author | Gondro, Cedric | en |
dc.contributor.author | Grounds, M D | en |
dc.date.accessioned | 2014-02-07T10:08:00Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | American Journal of Physiology: Cell Physiology, 305(1), p. C26-C35 | en |
dc.identifier.issn | 1522-1563 | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/13944 | - |
dc.description.abstract | Circadian rhythms and metabolism are tightly integrated, and rhythmic expression of metabolic factors is common in homeostatic processes. We measured the temporal changes in the expression of myogenic regulatory factors and expression and activity level of molecules involved in protein metabolism in skeletal muscles and livers in mice and examined the impact of fasting. Tissues were collected over 24 h (at zeitgeber times ZT1, ZT5, ZT9, ZT13, ZT17, ZT21, and ZT1 the following day) from adult male C57Bl/6J mice that had been either freely fed or fasted for 24 h. In skeletal muscle, there was a robust rise in the mRNA expression of the myogenic regulatory factors 'MyoD' and myogenin during dark hours which was strongly suppressed by fasting. Circadian pattern was observed for mRNA of 'MuRF1', 'Akt1', and 'ribosomal protein S6' in muscles in fed and fasted mice and for 'Fbxo32' in fed mice. Activity (phosphorylation) levels of Akt(Ser473) displayed temporal regulation in fasted (but not fed) mice and were high at ZT9. Fasting caused significant reductions in phosphorylation for both Akt and S6 in muscles, indicative of inactivation. Hepatic phosphorylated Akt(Ser473) and S6(Ser235/236) proteins did not exhibit daily rhythms. Fasting significantly reduced hepatic Akt(473) phosphorylation compared with fed levels, although (unlike in muscle) it did not affect S6(Ser235/236) phosphorylation. This in vivo circadian study addresses for the first time the signaling activities of key molecules related to protein turnover and their possible cross-regulation of expression of genes related to protein degradation. | en |
dc.language | en | en |
dc.publisher | American Physiological Society | en |
dc.relation.ispartof | American Journal of Physiology: Cell Physiology | en |
dc.title | Impact of fasting on the rhythmic expression of myogenic and metabolic factors in skeletal muscle of adult mice | en |
dc.type | Journal Article | en |
dc.identifier.doi | 10.1152/ajpcell.00027.2013 | en |
dcterms.accessRights | Gold | en |
dc.subject.keywords | Gene Expression (incl Microarray and other genome-wide approaches) | en |
local.contributor.firstname | T | en |
local.contributor.firstname | T | en |
local.contributor.firstname | Z | en |
local.contributor.firstname | G | en |
local.contributor.firstname | P J | en |
local.contributor.firstname | Cedric | en |
local.contributor.firstname | M D | en |
local.subject.for2008 | 060405 Gene Expression (incl Microarray and other genome-wide approaches) | en |
local.subject.seo2008 | 970106 Expanding Knowledge in the Biological Sciences | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.email | cgondro2@une.edu.au | en |
local.output.category | C1 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.identifier.epublicationsrecord | une-20130829-12388 | en |
local.publisher.place | United States of America | en |
local.format.startpage | C26 | en |
local.format.endpage | C35 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 305 | en |
local.identifier.issue | 1 | en |
local.access.fulltext | Yes | en |
local.contributor.lastname | Shavlakadze | en |
local.contributor.lastname | Anwari | en |
local.contributor.lastname | Soffe | en |
local.contributor.lastname | Cozens | en |
local.contributor.lastname | Mark | en |
local.contributor.lastname | Gondro | en |
local.contributor.lastname | Grounds | en |
dc.identifier.staff | une-id:cgondro2 | en |
local.profile.orcid | 0000-0003-0666-656X | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:14157 | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Impact of fasting on the rhythmic expression of myogenic and metabolic factors in skeletal muscle of adult mice | en |
local.output.categorydescription | C1 Refereed Article in a Scholarly Journal | en |
local.search.author | Shavlakadze, T | en |
local.search.author | Anwari, T | en |
local.search.author | Soffe, Z | en |
local.search.author | Cozens, G | en |
local.search.author | Mark, P J | en |
local.search.author | Gondro, Cedric | en |
local.search.author | Grounds, M D | en |
local.uneassociation | Unknown | en |
local.identifier.wosid | 000321197600004 | en |
local.year.published | 2013 | en |
local.subject.for2020 | 310505 Gene expression (incl. microarray and other genome-wide approaches) | en |
local.subject.seo2020 | 280102 Expanding knowledge in the biological sciences | en |
Appears in Collections: | Journal Article |
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