Please use this identifier to cite or link to this item:
https://hdl.handle.net/1959.11/11777
Title: | Multi-primer target PCR for rapid identification of bovine DRB3 alleles | Contributor(s): | Ledwidge, SA (author); Mallard, BA (author); Gibson, John Paul (author); Jansen, GB (author); Jiang, ZH (author) | Publication Date: | 2001 | DOI: | 10.1046/j.1365-2052.2001.00761.x | Handle Link: | https://hdl.handle.net/1959.11/11777 | Abstract: | Multi-primer target polymerase chain reaction (MPT-PCR) is a rapid method for the identification of specific BoLA-DRB3 alleles. In a single PCR reaction, the presence of two alleles associated with increased risk, DRB3.2*23 (DRB3*2701±2703, 2705±2707) and decreased risk, DRB3.2*16 (DRB3*1501, 1502), of mastitis in Canadian Holstein can be detected. Two outer primers amplify exon 2 of DRB3. Simultaneously, two inner, allele-specific primers amplify individual alleles. Initially, 40 cows previously typed by PCR-restriction fragment length polymorphism (PCR-RFLP) were genotyped using the multi-primer approach. An additional 30 cows were first genotyped by multi-primer target PCR, then by PCR-RFLP. All animals were correctly identified and there were no false positives. This technique can readily be modified to identify other BoLA alleles of interest. | Publication Type: | Journal Article | Source of Publication: | Animal Genetics, 32(4), p. 219-221 | Publisher: | Wiley-Blackwell Publishing Ltd | Place of Publication: | United Kingdom | ISSN: | 1365-2052 0268-9146 |
Fields of Research (FoR) 2008: | 070201 Animal Breeding | Socio-Economic Objective (SEO) 2008: | 830301 Beef Cattle | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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