Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/10046
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dc.contributor.authorMoens, Pierreen
dc.contributor.authorKrishnan, Kannanen
dc.contributor.authorBagatolli, Luis Aen
dc.date.accessioned2012-05-02T17:14:00Z-
dc.date.issued2008-
dc.identifier.citationBiophysical Journal, 94(2, Supplement), p. 691-691en
dc.identifier.issn1542-0086en
dc.identifier.issn0006-3495en
dc.identifier.urihttps://hdl.handle.net/1959.11/10046-
dc.description.abstractProfilin is a small (12-15 kDa) actin binding protein. Profilin is also involved in the signaling pathway linking receptors in the cell membrane to the microfilament system within the cell. Profilin is thought to play critical roles in this signaling pathway through its interaction with polyphosphoinositides (PPI). To date, profilin's interaction with PPI has only been studied in micelles or small vesicles. Profilin binds with high affinity to small clusters of phosphatidylinositol (4, 5) bis-phosphate [PI(4, 5)P₂] molecules. In this work, we investigated the interactions of profilin with sub-micellar concentrations of PI(4, 5)P₂ and PI(3, 4, 5)P₃. Fluorescence anisotropy was used to determine the relevant dissociation constants for binding of sub-micellar concentrations of fluorescently labeled PPI lipids to profilin and we show that these are significantly different from those determined for profilin interaction with micelles or small vesicles. Despite the low affinity for submicellar concentration of PI(4, 5)P₂, profilin was shown to bind to Giant unilamellar vesicles in presence of 1% mole fraction of PI(4, 5)P₂ Forster Resonance Energy Transfer experiments indicate that profilin is able to recruit adjacent PI(4, 5)P₂ molecules after initial interaction with the GUV membrane. The implications of these findings are discussed.en
dc.languageenen
dc.publisherCell Pressen
dc.relation.ispartofBiophysical Journalen
dc.relation.ispartofseriesBiophysical Society Annual Meeting Abstractsen
dc.titleInteractions Between Profilin And Polyphosphatidylinositol Lipidsen
dc.typeConference Publicationen
dc.relation.conferenceBPS Annual Meeting/IUPAB 2008: 52nd Annual Meeting of the Biophysical Society and 16th International Biophysics Congressen
dc.identifier.doi10.1016/S0006-3495(08)79122-7en
dc.subject.keywordsBiological Physicsen
dc.subject.keywordsAnalytical Biochemistryen
local.contributor.firstnamePierreen
local.contributor.firstnameKannanen
local.contributor.firstnameLuis Aen
local.subject.for2008060101 Analytical Biochemistryen
local.subject.for2008029901 Biological Physicsen
local.subject.seo2008970106 Expanding Knowledge in the Biological Sciencesen
local.profile.schoolSchool of Science and Technologyen
local.profile.schoolHuman Biology and Physiologyen
local.profile.schoolHuman Biology and Physiologyen
local.profile.emailpmoens@une.edu.auen
local.profile.emailkkrishna@une.edu.auen
local.output.categoryE3en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20120430-172317en
local.date.conference2nd - 6th February, 2008en
local.conference.placeLong Beach, United States of Americaen
local.publisher.placeUnited States of Americaen
local.identifier.runningnumberPresentation Number: 2060-Posen
local.format.startpage691en
local.format.endpage691en
local.series.number2008en
local.identifier.volume94en
local.identifier.issue2, Supplementen
local.contributor.lastnameMoensen
local.contributor.lastnameKrishnanen
local.contributor.lastnameBagatollien
dc.identifier.staffune-id:pmoensen
dc.identifier.staffune-id:kkrishnaen
local.profile.orcid0000-0003-3121-5306en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:10237en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleInteractions Between Profilin And Polyphosphatidylinositol Lipidsen
local.output.categorydescriptionE3 Extract of Scholarly Conference Publicationen
local.relation.urlhttp://www.biophysics.org/tabid/461/default.aspxen
local.conference.detailsBPS Annual Meeting/IUPAB 2008: 52nd Annual Meeting of the Biophysical Society and 16th International Biophysics Congress, Long Beach, United States of America, 2nd - 6th February, 2008en
local.search.authorMoens, Pierreen
local.search.authorKrishnan, Kannanen
local.search.authorBagatolli, Luis Aen
local.uneassociationUnknownen
local.year.published2008en
local.date.start2008-02-02-
local.date.end2008-02-06-
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