Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/10045
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dc.contributor.authorMoens, Pierreen
dc.contributor.authorWahl, Marcusen
dc.contributor.authorJameson, David Michaelen
dc.date.accessioned2012-05-02T16:59:00Z
dc.date.issued2004en
dc.identifier.citationBiophysical Journal, 86(Supplement 1, Part 2), p. 82a-83aen
dc.identifier.issn0006-3495en
dc.identifier.urihttps://hdl.handle.net/1959.11/10045en
dc.description.abstractThe "stalk" of the prokaryotic 50S ribosomal subunit contains four copies of the protein L7/L12. In 'E. Coli', L7/L12 is a dimeric protein, which is able to undergo rapid subunit exchange (Hamman et al., 1996 Biochemistry 35: 16680-6). Using fluorescence polarization we investigated subunit exchange between labeled cysteine mutants of 'T. maritima' L12 and wild type proteins. In these systems, polarization increases after subunit exchange due to relief of depolarization caused by homo-energy transfer. We demonstrated that the rate of subunit exchange in 'T. maritima' L12 is significantly slower at 25C than in the 'E. coli' system. We also show that in 'T. maritima' the exchange rate increases with increasing temperature. Possible factors responsible for the difference between these two systems are discussed. We also investigated the mode of interactions between L12 subunits. The crystal structure suggested a tetrameric arrangement of the L12 proteins from 'T. maritima' (Wahl, et al., 2000 Embo J 19, 174-86). In our solution studies, however, data from Forster Resonance Energy Transfer experiments supported a dimer model for L12. From the X-ray data, 2 possible dimerization modes were proposed. In the first mode, the monomers form a tight symmetric and parallel dimer. In the second mode, the monomers bind through their N-terminal region in an anti-parallel configuration with one monomer in a tight conformation and the other monomer adopting an elongated shape. Our results support the second mode. These studies were supported by NSF grant MCB9808427 (DMJ).en
dc.languageenen
dc.publisherCell Pressen
dc.relation.ispartofBiophysical Journalen
dc.relation.ispartofseriesBiophysical Society Annual Meeting Abstractsen
dc.titleFluorescence Studies Of Subunit Exchange In 'Thermotoga Maritima' Ribosomal Stalk Protein L12en
dc.typeConference Publicationen
dc.relation.conference48th Annual Meeting of the Biophysical Society, Baltimore, United States of America, 14th - 18th February, 2004en
dc.subject.keywordsBiological Physicsen
dc.subject.keywordsAnalytical Biochemistryen
local.contributor.firstnamePierreen
local.contributor.firstnameMarcusen
local.contributor.firstnameDavid Michaelen
local.subject.for2008029901 Biological Physicsen
local.subject.for2008060101 Analytical Biochemistryen
local.subject.seo2008970106 Expanding Knowledge in the Biological Sciencesen
local.profile.schoolSchool of Science and Technologyen
local.profile.schoolHuman Biology and Physiologyen
local.profile.schoolHuman Biology and Physiologyen
local.profile.emailpmoens@une.edu.auen
local.output.categoryE3en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20120430-140917en
local.publisher.placeUnited States of Americaen
local.identifier.runningnumberPresentation Number: 427-Posen
local.format.startpage82aen
local.format.endpage83aen
local.series.issn0523-6800en
local.series.number2004en
local.identifier.volume86en
local.identifier.issueSupplement 1, Part 2en
local.contributor.lastnameMoensen
local.contributor.lastnameWahlen
local.contributor.lastnameJamesonen
dc.identifier.staffune-id:pmoensen
local.profile.orcid0000-0003-3121-5306en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:10236en
dc.identifier.academiclevelAcademicen
local.title.maintitleFluorescence Studies Of Subunit Exchange In 'Thermotoga Maritima' Ribosomal Stalk Protein L12en
local.output.categorydescriptionE3 Extract of Scholarly Conference Publicationen
local.relation.urlhttp://www.biophysics.org/tabid/461/default.aspxen
local.conference.details48th Annual Meeting of the Biophysical Society, Baltimore, United States of America, 14th - 18th February, 2004en
local.description.statisticsepubsVisitors: 173<br />Views: 172<br />Downloads: 0en
local.search.authorMoens, Pierreen
local.search.authorWahl, Marcusen
local.search.authorJameson, David Michaelen
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