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https://hdl.handle.net/1959.11/10044
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DC Field | Value | Language |
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dc.contributor.author | Moens, Pierre | en |
dc.contributor.author | Wahl, Marcus | en |
dc.contributor.author | Jameson, David Michael | en |
local.source.editor | Editor(s): Julian White and Vaughan K Williams | en |
dc.date.accessioned | 2012-05-02T16:22:00Z | - |
dc.date.issued | 2003 | - |
dc.identifier.citation | 14th World Congress on Animal, Plant & Microbial Toxins Abstract and Programme Book, p. 127-127 | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/10044 | - |
dc.description.abstract | One of the most characteristic structural features of the prokaryotic 50S ribosomal subunit is the "stalk", a protuberance that contains the protein called L7/L12. In 'E. Coli', L7/L12 is a dimeric protein which is able to undergo rapid subunit exchange (Hamman et al., 1996 Biochemistry 35: 16680-6,). Recent X-ray data reported a tetrameric arrangement of the L12 proteins isolated from 'Termotoga Maritima'. This structure was used to propose 2 alternative dimerization modes. In one mode, the 2 monomers of L12 form a tight symmetric and parallel dimer held together by a four helix bundle. In the other mode, the two monomers bind through their N-terminal region in an anti-parallel configuration with one monomer in a tight conformation and the other monomer adopting an elongated shape. The first mode of dimerization has been used to represent L7/L12 into the structural model of the 70S ribosome and a recent publication (Nomura et al., 2003 Biochemistry 42: 4691-8) used this mode to discuss their experimental results. In this work, we present data from Forster Resonance Energy Transfer experiments using labelled cysteine mutants of 'T. maritima', which support the second mode of dimerization, i.e. in an anti-parallel orientation and an elongated configuration. We also demonstrate that the rate of subunit exchange among a population of 'T. maritima' LI2 is significantly slower than in the 'E. coli' system. | en |
dc.language | en | en |
dc.publisher | International Society on Toxinology | en |
dc.relation.ispartof | 14th World Congress on Animal, Plant & Microbial Toxins Abstract and Programme Book | en |
dc.title | Dimerization mode of 'T. maritima' ribosomal stalk proteins L12 in solution | en |
dc.type | Conference Publication | en |
dc.relation.conference | Toxicon 2003: 14th World Congress on Animal, Plant & Microbial Toxins | en |
dc.subject.keywords | Biological Physics | en |
dc.subject.keywords | Analytical Biochemistry | en |
local.contributor.firstname | Pierre | en |
local.contributor.firstname | Marcus | en |
local.contributor.firstname | David Michael | en |
local.subject.for2008 | 060101 Analytical Biochemistry | en |
local.subject.for2008 | 029901 Biological Physics | en |
local.subject.seo2008 | 970106 Expanding Knowledge in the Biological Sciences | en |
local.profile.school | School of Science and Technology | en |
local.profile.school | Human Biology and Physiology | en |
local.profile.school | Human Biology and Physiology | en |
local.profile.email | pmoens@une.edu.au | en |
local.output.category | E3 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.identifier.epublicationsrecord | une-20120430-135747 | en |
local.date.conference | 14th - 19th September, 2003 | en |
local.conference.place | Adelaide, Australia | en |
local.publisher.place | Australia | en |
local.identifier.runningnumber | ASB Abstract Number ASB05 | en |
local.format.startpage | 127 | en |
local.format.endpage | 127 | en |
local.contributor.lastname | Moens | en |
local.contributor.lastname | Wahl | en |
local.contributor.lastname | Jameson | en |
dc.identifier.staff | une-id:pmoens | en |
local.profile.orcid | 0000-0003-3121-5306 | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:10235 | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Dimerization mode of 'T. maritima' ribosomal stalk proteins L12 in solution | en |
local.output.categorydescription | E3 Extract of Scholarly Conference Publication | en |
local.relation.url | http://handle.dtic.mil/100.2/ADA418029 | en |
local.conference.details | Toxicon 2003: 14th World Congress on Animal, Plant & Microbial Toxins, Adelaide, Australia, 14th - 19th September, 2003 | en |
local.search.author | Moens, Pierre | en |
local.search.author | Wahl, Marcus | en |
local.search.author | Jameson, David Michael | en |
local.uneassociation | Unknown | en |
local.year.published | 2003 | en |
local.date.start | 2003-09-14 | - |
local.date.end | 2003-09-19 | - |
Appears in Collections: | Conference Publication |
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