Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/10042
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dc.contributor.authorKrishnan, Kannanen
dc.contributor.authorCody, Stephen Hen
dc.contributor.authorClayton, Andrew H Aen
dc.contributor.authorMoens, Pierreen
dc.date.accessioned2012-05-02T15:21:00Z-
dc.date.issued2007-
dc.identifier.citationProceedings of the Australian Physiological Society, v.38, p. 8-8en
dc.identifier.issn0067-2084en
dc.identifier.urihttps://hdl.handle.net/1959.11/10042-
dc.description.abstractHuman Profilin-I is a small actin binding proteins which also interact with polyphosphoinositides (PPI) (Lassing & Lindberg, 1985) and proline rich motif containing proteins. Profilin is involved in the signaling pathway linking the receptors in the cell membrane to the microfilament system. Profilin is thought to play critical roles in this signaling pathway through its interaction with phosphatidylinositol 4,5-bisphosphate [PI(4,5)P₂] (Lu et al., 1996). So far, profilin's interaction with PPI has only been studied in micelles or small vesicles. Profilin binds with high affinity to small clusters of PI(4,5)P₂ molecules (Goldschmidt-Clermont et al., 1991). In cells, the organization of PPI lipids is different from micelles, therefore the interaction with profilin might be quite different. We have used giant unilamellar vesicles (GUV) as a membrane model system to investigate the interactions between PI(4,5)P₂ and profilin. BODIPY® TMR PI(4,5)P₂C16 (Rhodamine labelled 16-carbon fatty acid containing Phosphatidylinositol 4, 5-bisphosphate) was incorporated into GUV membrane. Confocal images of GUVs were obtained in presence and absence of profilin. These images were analysed using SimFCS software (Prof. Enrico Gratton, Laboratory for Fluorescence Dynamics, Irvine, USA). The diffusion coefficient and the aggregation of PIP2 in the membrane were determined and profilin had a clear effect upon the diffusion coefficient and aggregation of PIP2.en
dc.languageenen
dc.publisherAustralian Physiological Society (AuPS)en
dc.relation.ispartofProceedings of the Australian Physiological Societyen
dc.relation.ispartofseriesProceedings of the Australian Physiological Societyen
dc.titleInteractions of human profilin-1 and phosphatidylinositol 4,5-bisphosphate in giant unilamellar vesiclesen
dc.typeConference Publicationen
dc.relation.conferenceAuPS/ASB 2007: Joint Annual Meeting of the Australian Physiological Society and Australian Society for Biophysicsen
dc.subject.keywordsBiological Physicsen
dc.subject.keywordsAnalytical Biochemistryen
local.contributor.firstnameKannanen
local.contributor.firstnameStephen Hen
local.contributor.firstnameAndrew H Aen
local.contributor.firstnamePierreen
local.subject.for2008060101 Analytical Biochemistryen
local.subject.for2008029901 Biological Physicsen
local.subject.seo2008970106 Expanding Knowledge in the Biological Sciencesen
local.profile.schoolSchool of Science and Technologyen
local.profile.emailkkrishna@une.edu.auen
local.profile.emailpmoens@une.edu.auen
local.output.categoryE3en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20120430-174314en
local.date.conference2nd - 5th December, 2007en
local.conference.placeNewcastle, Australiaen
local.publisher.placeAustraliaen
local.format.startpage8en
local.format.endpage8en
local.series.issn0067-2084en
local.series.number38en
local.identifier.volume38en
local.contributor.lastnameKrishnanen
local.contributor.lastnameCodyen
local.contributor.lastnameClaytonen
local.contributor.lastnameMoensen
dc.identifier.staffune-id:kkrishnaen
dc.identifier.staffune-id:pmoensen
local.profile.orcid0000-0003-3121-5306en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:10233en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleInteractions of human profilin-1 and phosphatidylinositol 4,5-bisphosphate in giant unilamellar vesiclesen
local.output.categorydescriptionE3 Extract of Scholarly Conference Publicationen
local.relation.urlhttp://aups.org.au/Proceedings/38/8P/en
local.conference.detailsAuPS/ASB 2007: Joint Annual Meeting of the Australian Physiological Society and Australian Society for Biophysics, Newcastle, Australia, 2nd - 5th December, 2007en
local.search.authorKrishnan, Kannanen
local.search.authorCody, Stephen Hen
local.search.authorClayton, Andrew H Aen
local.search.authorMoens, Pierreen
local.uneassociationUnknownen
local.year.published2007-
local.date.start2007-12-02-
local.date.end2007-12-05-
local.profile.affiliationtypeUnknownen
local.profile.affiliationtypeUnknownen
local.profile.affiliationtypeUnknownen
local.profile.affiliationtypeUnknownen
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