Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/9223
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dc.contributor.authorHou, Xingshengen
dc.contributor.authorPereg, Lilyen
dc.contributor.authorWatson, Kennethen
dc.date.accessioned2012-01-16T16:48:00Z
dc.date.created2009en
dc.date.issued2010en
dc.identifier.urihttps://hdl.handle.net/1959.11/9223en
dc.description.abstractIn 'Azospirillum brasilense', a possible transcriptional regulator, FlcA, controls the differentiation of cells from vegetative to cyst forms. In this study, a suicide plasmid pSUP-flcAΔ was constructed, and by homologous recombination between the plasmid and the genomic DNA of 'Azospirillum brasilense' Sp7, the open reading frame of flcA was deleted in the genome of wild-type Sp7. In contrast to wild-type 'A. brasilense' Sp7, this flcA in-frame knock-out strain, named Sp7-flcAΔ, did not aggregate and remained in vegetative form under flocculation conditions. These FlcA deficient bacteria binded weakly Congo-red, lacked an extra-cellular polysaccharides coat on their cell surface and lacked poly-β-hydroxybutyrate granules within the cells under flocculation conditions. Compared to the wild-type Sp7, Sp7-flcAΔ weakly attached to wheat roots, and showed a significant reduction of survival rates under stress conditions, like UV radiation, heat and osmotic stress.en
dc.languageenen
dc.titleFlcA regulation of metabolism and stress response in 'Azospirillum brasilense' Sp7en
dc.typeThesis Doctoralen
dc.subject.keywordsMicrobial Geneticsen
local.contributor.firstnameXingshengen
local.contributor.firstnameLilyen
local.contributor.firstnameKennethen
local.subject.for2008060503 Microbial Geneticsen
local.subject.seo2008970106 Expanding Knowledge in the Biological Sciencesen
local.subject.seo780105 Biological Sciencesden
dcterms.RightsStatementCopyright 2009 - Xingsheng Houen
dc.date.conferred2010en
local.thesis.degreelevelDoctoralen
local.thesis.degreenameDoctor of Philosophyen
local.contributor.grantorUniversity of New Englanden
local.profile.schoolSchool of Science and Technologyen
local.profile.schoolSchool of Science and Technologyen
local.profile.schoolAdministrationen
local.profile.emailxhou@une.edu.auen
local.profile.emaillperegge@une.edu.auen
local.profile.emailkwatson2@une.edu.auen
local.output.categoryT2en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune_thesis-20090507-08027en
local.contributor.lastnameHouen
local.contributor.lastnamePeregen
local.contributor.lastnameWatsonen
dc.identifier.staffune-id:xhouen
dc.identifier.staffune-id:lpereggeen
dc.identifier.staffune-id:kwatson2en
local.profile.roleauthoren
local.profile.rolesupervisoren
local.profile.rolesupervisoren
local.identifier.unepublicationidune:9414en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleFlcA regulation of metabolism and stress response in 'Azospirillum brasilense' Sp7en
local.output.categorydescriptionT2 Thesis - Doctorate by Researchen
local.description.statisticsepubsVisitors: 72<br />Views: 107<br />Downloads: 4en
local.thesis.borndigitalyesen
local.search.authorHou, Xingshengen
local.search.supervisorPereg, Lilyen
local.search.supervisorWatson, Kennethen
Appears in Collections:Thesis Doctoral
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