Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/8980
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dc.contributor.authorNgo, Suong Ngoc Thien
dc.contributor.authorMcKinnon, Ross Allanen
dc.contributor.authorStupans, Ievaen
dc.date.accessioned2011-12-05T09:11:00Z-
dc.date.issued2007-
dc.identifier.citationComparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 146(3), p. 375-382en
dc.identifier.issn1878-1659en
dc.identifier.issn1532-0456en
dc.identifier.urihttps://hdl.handle.net/1959.11/8980-
dc.description.abstractPeroxisome proliferator-activated receptor α (PPARα) is a member of the nuclear/steroid receptor gene superfamily that plays an essential role in fatty acid metabolism. PPARα modulates the expression of genes encoding peroxisomal fatty acid β-oxidation enzymes and microsomal fatty acid hydroxylases CYP4As. We have previously reported that the obligate 'Eucalyptus' feeder koala ('Phascolarctos cinereus') exhibits a higher hepatic CYP4A activity and an absence of peroxisomal palmitoyl-CoA oxidation as compared to non-Eucalyptus feeders human, rat or wallaby. Here we describe the cloning, expression and molecular characterisation of koala hepatic PPARα. A full-length PPARα cDNA of size 1515 bp was cloned by reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The koala PPARα cDNA encodes a protein of 468 amino acids. Transfection of the koala PPARα cDNA into Cos-7 cells resulted in the expression of a protein recognised by a rabbit anti-human PPARα polyclonal antibody. PPARα immunoreactive bands of the same molecular mass were detected in nuclear extracts of koala livers. The results of this study demonstrate the presence of koala hepatic PPARα which shares several common features with other published PPARαs; however, it exhibits important differences in both the DNA and ligand binding domains.en
dc.languageenen
dc.publisherElsevier Incen
dc.relation.ispartofComparative Biochemistry and Physiology Part C: Toxicology & Pharmacologyen
dc.titleHepatic nuclear receptor PPARα in the koala ('Phascolarctos cinereus'): Cloning and molecular characterisationen
dc.typeJournal Articleen
dc.identifier.doi10.1016/j.cbpc.2007.04.013en
dc.subject.keywordsMedical and Health Sciencesen
local.contributor.firstnameSuong Ngoc Thien
local.contributor.firstnameRoss Allanen
local.contributor.firstnameIevaen
local.subject.for2008119999 Medical and Health Sciences not elsewhere classifieden
local.subject.seo2008929999 Health not elsewhere classifieden
local.profile.schoolSchool of Science and Technologyen
local.profile.emailistupans@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20111202-092019en
local.publisher.placeUnited States of Americaen
local.format.startpage375en
local.format.endpage382en
local.peerreviewedYesen
local.identifier.volume146en
local.identifier.issue3en
local.title.subtitleCloning and molecular characterisationen
local.contributor.lastnameNgoen
local.contributor.lastnameMcKinnonen
local.contributor.lastnameStupansen
dc.identifier.staffune-id:istupansen
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:9170en
dc.identifier.academiclevelAcademicen
local.title.maintitleHepatic nuclear receptor PPARα in the koala ('Phascolarctos cinereus')en
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.search.authorNgo, Suong Ngoc Thien
local.search.authorMcKinnon, Ross Allanen
local.search.authorStupans, Ievaen
local.uneassociationUnknownen
local.year.published2007en
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