Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/8954
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dc.contributor.authorJones, Brett Ren
dc.contributor.authorEl-Merhibi, Adaweyahen
dc.contributor.authorNgo, Suong NTen
dc.contributor.authorStupans, Ievaen
dc.contributor.authorMcKinnon, Ross Aen
dc.date.accessioned2011-11-30T17:33:00Z-
dc.date.issued2008-
dc.identifier.citationComparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 148(3), p. 230-237en
dc.identifier.issn1878-1659en
dc.identifier.issn1532-0456en
dc.identifier.urihttps://hdl.handle.net/1959.11/8954-
dc.description.abstractCytochromes P450 (CYPs) are critically important in the oxidative metabolism of a diverse array of xenobiotics and endogenous substrates. We have previously reported that the obligate 'Eucalyptus' feeder koala ('Phascolarctos cinereus') exhibits a higher hepatic CYP2C activity as compared to non-Eucalyptus feeders human or rat, with stimulation of CYP2C activity by cineole. In the present study, we examine CYP2C expression by immunohistochemistry and describe the identification and cloning of koala CYP2Cs. Utilising anti-rat CYP2C6 antibody, the expression of CYP2C was found to be uniform across the hepatic sections, being consistent with that observed in human and rat. Two 1647 and 1638 bp koala liver CYP2C complete cDNAs, designated CYP2C47 and CYP2C48 respectively, were cloned by cDNA library screening. The koala CYP2C cDNAs encode a protein of 495 amino acids. Three additional partial CYP2C sequences were also identified from the koala, indicating the multiplicity of the CYP2C subfamily in this unique marsupial species. The results of this study demonstrate the presence of koala hepatic CYP2Cs that share several common features with other published CYP2Cs; however CYP2C47 and CYP2C48 contain four extra amino acid residues at the NH2-terminal, a transmembrane anchor which was reported being a fundamentally conserved structure core of all eukaryote CYP enzymes.en
dc.languageenen
dc.publisherElsevier Incen
dc.relation.ispartofComparative Biochemistry and Physiology Part C: Toxicology & Pharmacologyen
dc.titleHepatic cytochrome P450 enzymes belonging to the CYP2C subfamily from an Australian marsupial, the koala ('Phascolarctos cinereus')en
dc.typeJournal Articleen
dc.identifier.doi10.1016/j.cbpc.2008.05.020en
dc.subject.keywordsMedical and Health Sciencesen
local.contributor.firstnameBrett Ren
local.contributor.firstnameAdaweyahen
local.contributor.firstnameSuong NTen
local.contributor.firstnameIevaen
local.contributor.firstnameRoss Aen
local.subject.for2008119999 Medical and Health Sciences not elsewhere classifieden
local.subject.seo2008929999 Health not elsewhere classifieden
local.profile.schoolSchool of Science and Technologyen
local.profile.emailistupans@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20111130-143936en
local.publisher.placeUnited States of Americaen
local.format.startpage230en
local.format.endpage237en
local.peerreviewedYesen
local.identifier.volume148en
local.identifier.issue3en
local.contributor.lastnameJonesen
local.contributor.lastnameEl-Merhibien
local.contributor.lastnameNgoen
local.contributor.lastnameStupansen
local.contributor.lastnameMcKinnonen
dc.identifier.staffune-id:istupansen
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:9144en
dc.identifier.academiclevelAcademicen
local.title.maintitleHepatic cytochrome P450 enzymes belonging to the CYP2C subfamily from an Australian marsupial, the koala ('Phascolarctos cinereus')en
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.search.authorJones, Brett Ren
local.search.authorEl-Merhibi, Adaweyahen
local.search.authorNgo, Suong NTen
local.search.authorStupans, Ievaen
local.search.authorMcKinnon, Ross Aen
local.uneassociationUnknownen
local.year.published2008en
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