Please use this identifier to cite or link to this item:
|Title:||Cloning and expression of koala ('Phascolarctos cinereus') liver cytochrome P450 reductase||Contributor(s):||Kong, Sandra (author); Ngo, Suong NT (author); McKinnon, Ross A (author); Stupans, Ieva (author)||Publication Date:||2009||DOI:||10.1016/j.cbpc.2009.01.010||Handle Link:||https://hdl.handle.net/1959.11/8952||Abstract:||The cloning, expression and characterization of hepatic NADPH-cytochrome P450 reductase (CPR) from koala ('Phascolarctos cinereus') is described. Two 2059 bp koala liver CPR cDNAs, designated CPR1 and CPR2, were cloned by reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends. The koala CPR cDNAs encode proteins of 678 amino acids and share 85% amino acid sequence identity to human CPR. Transfection of the koala CPR cDNAs into Cos-7 cells resulted in the expression of proteins, which were recognized by a goat-antihuman CPR antibody. The koala CPR1 and 2 cDNA-expressed enzymes catalysed cytochrome c reductase at the rates of 4.9 ± 0.5 and 2.6 ± 0.4 nmol/min/mg protein (mean ± SD, n = 3), respectively which were comparable to that of rat CPR cDNA-expressed enzyme. The apparent Km value for CPR activity in koala liver microsomes was 11.61 ± 6.01 µM, which is consistent with that reported for rat CPR enzyme. Northern analysis detected a CPR mRNA band of approximately 2.6 kb. Southern analysis suggested a single PCR gene across species. The present study provides primary molecular data regarding koala CPR1 and CPR2 genes in this unique marsupial species.||Publication Type:||Journal Article||Source of Publication:||Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 150(1), p. 1-9||Publisher:||Elsevier Inc||Place of Publication:||United States of America||ISSN:||1878-1659
|Field of Research (FOR):||119999 Medical and Health Sciences not elsewhere classified||Socio-Economic Outcome Codes:||929999 Health not elsewhere classified||Peer Reviewed:||Yes||HERDC Category Description:||C1 Refereed Article in a Scholarly Journal||Statistics to Oct 2018:||Visitors: 77
|Appears in Collections:||Journal Article|
Files in This Item:
checked on Nov 30, 2018
checked on Dec 29, 2018
Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.