Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/8733
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dc.contributor.authorMcMillen, I Cen
dc.contributor.authorHoughton, D Cen
dc.contributor.authorMcFarlane, James Ren
dc.contributor.authorKauter, Kathleenen
dc.contributor.authorYoung, I Ren
dc.date.accessioned2011-10-26T09:20:00Z-
dc.date.issued2004-
dc.identifier.citationJournal of the Society for Gynecologic Investigation, 11(2: Supplement), p. 111A-111Aen
dc.identifier.issn1071-5576en
dc.identifier.issn1933-7205en
dc.identifier.issn1933-7191en
dc.identifier.urihttps://hdl.handle.net/1959.11/8733-
dc.description.abstractObjective: In the adult sheep, leptin synthesis and secretion in adipose tissue is regulated by a range of factors including the external photoperiod, hypothalamic neuropeptides and peripheral nutrient and hormonal signals. While it has been established that leptin is synthesised in adipose tissue and is present in the circulation before birth, it is not known whether the external photoperiod or fetal hypothalamus plays a role in the regulation of leptin synthesis or secretion in late gestation. We have utilised a model of hypothalamo-pituitary disconnection (HPD) in which the neural tissues of the median eminence and surrounding arcuate nucleus are removed, and a physical barrier is inserted above the pars tuberalis to separate the hypothalamus and pituitary to investigate the role of the hypothalamus in the tonic regulation of fetal leptin in ewes held under long light (LL:1 6h L:8h D) or short light (8hL:l6hD) conditions. Methods: Twenty three ewes were housed in a I2hL:12D regime until surgery between 106 and 120d gestation.HPD was performed in the fetuses of 10 ewes and a sham procedure carried out in 13 fetuses. Six ewes (HPD group) and 6 ewes (intact group) were moved into a LL regime, whereas 4 ewes (HPD group) and 7 ewes (intact group) were moved into a SL regime. Maternal and fetal blood samples were collected throughout late gestation until 142d gestation. The completeness of the HPD procedure was confirmed using the lack of a proalctin response to a chlorpromazine challenge as previously described. Plasma leptin concentrations were measured using an ELISA and the effects of photoperiod and HPD on leptin concentrations were determined using a multifactorial Analysis of Variance and repeated measures. Results: In the ewes, there was no effect of varying the length of the photoperiod or of carrying an HPD fetus on maternal plasma concentrations of leptin. There was also no effect of external photoperiod on fetal plasma leptin concentrations. Fetal plasma leptin concentrations were significantly higher, however, in the HPD fetal sheep (LL, 5.9 ± 1.3 ng/ml: SL, 7.9 ± 2.6 ng/ml) than in the intact group (LL, 4.7 ± 0.8 ng/ml: SL, 4.0 ± 1.4 ng/ml) throughout late gestation Conclusion: We have demonstrated that surgical disconnection of the fetal hypothalamus and the pituitary results in an increase in circulating leptin throughout late gestation. This suggests that either the mediobasal region of the fetal hypothalamus or the fetal pituitary act to inhibit leptin synthesis or secretion throughout late gestation.en
dc.languageenen
dc.publisherSociety for Gynecologic Investigationen
dc.relation.ispartofJournal of the Society for Gynecologic Investigationen
dc.titleEvidence for hypothalamic control of fetal leptin secretion during late gestationen
dc.typeJournal Articleen
dc.subject.keywordsReproductionen
dc.subject.keywordsEndocrinologyen
dc.subject.keywordsMetabolic Medicineen
local.contributor.firstnameI Cen
local.contributor.firstnameD Cen
local.contributor.firstnameJames Ren
local.contributor.firstnameKathleenen
local.contributor.firstnameI Ren
local.subject.for2008110306 Endocrinologyen
local.subject.for2008111404 Reproductionen
local.subject.for2008110107 Metabolic Medicineen
local.subject.seo2008920106 Endocrine Organs and Diseases (excl. Diabetes)en
local.subject.seo2008920114 Reproductive System and Disordersen
local.profile.schoolSchool of Science and Technologyen
local.profile.schoolSchool of Science and Technologyen
local.profile.emailjmcfarla@une.edu.auen
local.profile.emailkkauter@une.edu.auen
local.output.categoryC4en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordpes:7089en
local.publisher.placeUnited States of Americaen
local.identifier.runningnumberScientific Abstract 120en
local.format.startpage111Aen
local.format.endpage111Aen
local.identifier.volume11en
local.identifier.issue2: Supplementen
local.contributor.lastnameMcMillenen
local.contributor.lastnameHoughtonen
local.contributor.lastnameMcFarlaneen
local.contributor.lastnameKauteren
local.contributor.lastnameYoungen
dc.identifier.staffune-id:jmcfarlaen
dc.identifier.staffune-id:kkauter3en
local.profile.orcid0000-0003-4429-5384en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:8923en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleEvidence for hypothalamic control of fetal leptin secretion during late gestationen
local.output.categorydescriptionC4 Letter of Noteen
local.relation.urlhttp://rsx.sagepub.com/content/11/2_supplen
local.search.authorMcMillen, I Cen
local.search.authorHoughton, D Cen
local.search.authorMcFarlane, James Ren
local.search.authorKauter, Kathleenen
local.search.authorYoung, I Ren
local.uneassociationUnknownen
local.year.published2004en
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