Comparison of alternatives to in-feed antimicrobials for the prevention of clinical necrotic enteritis

Geier, M; Mikkelsen, Lene Lind; Torok, V A; Allison, Glenys M; Olnood, C G; Boulianne, N; Hughes, R J; Choct, Mingan

doi:10.1111/j.1365-2672.2010.04758.x

Author(s)

Geier, M

Mikkelsen, Lene Lind

Torok, V A

Allison, Glenys M

Olnood, C G

Boulianne, N

Hughes, R J

Choct, Mingan

Publication Date

2010

Abstract

Aims: The capacity for 'Lactobacillus johnsonii' and an organic acid (OA) blend to prevent 'Clostridium perfringens'-induced clinical necrotic enteritis (NE) in chickens was studied. Methods and Results: Cobb 500 birds were allocated into six groups (n = 25 birds/pen, eight pens/treatment); Unchallenged, Challenged, Antimicrobial (zinc bacitracin (ZnB)/monensin), OA, probiotic 'Lact. johnsonii' and probiotic sham (Phosphate–buffered saline). All birds were challenged with 'Eimeria' spp. and 'Cl. perfringens' except for unchallenged controls. Birds fed antimicrobials were protected from NE development as indicated by maintenance of body weight, low mortality and clostridium levels, and decreased intestinal macroscopic lesion scores compared to challenged controls (P < 0·05). 'Lactobacillus johnsonii'-fed birds had reduced lesion scores, whilst OA-fed birds had decreased 'Cl. perfringens' levels. Both 'Lact. johnsonii' and OA-fed birds had improved feed efficiency between days 0 and 28 compared to challenged controls; however, mortality and body weights were not improved by either treatment. Microbial profiling indicated that the challenge procedure significantly altered the jejunal microbiota. The microbiota of antimicrobial-fed birds was significantly different from all other groups. Conclusions: Whilst 'Lact. johnsonii' and OA altered specific intestinal parameters, significant protection against NE was not observed. Significance and Impact of the Study: 'Lactobacillus johnsonii' and OA did not prevent NE; however, some improvements were evident. Other related treatments, or combinations of these two treatments, may provide greater protection.

Citation

Journal of Applied Microbiology, 109(4), p. 1329-1338

ISSN

1365-2672

1364-5072

Link

https://hdl.handle.net/1959.11/7758

Language

en

Publisher

Wiley-Blackwell Publishing Ltd

Title

Comparison of alternatives to in-feed antimicrobials for the prevention of clinical necrotic enteritis

Type of document

Journal Article

Entity Type

Publication

Author(s)	Geier, M Mikkelsen, Lene Lind Torok, V A Allison, Glenys M Olnood, C G Boulianne, N Hughes, R J Choct, Mingan
Publication Date	2010
Abstract	Aims: The capacity for 'Lactobacillus johnsonii' and an organic acid (OA) blend to prevent 'Clostridium perfringens'-induced clinical necrotic enteritis (NE) in chickens was studied. Methods and Results: Cobb 500 birds were allocated into six groups (n = 25 birds/pen, eight pens/treatment); Unchallenged, Challenged, Antimicrobial (zinc bacitracin (ZnB)/monensin), OA, probiotic 'Lact. johnsonii' and probiotic sham (Phosphate–buffered saline). All birds were challenged with 'Eimeria' spp. and 'Cl. perfringens' except for unchallenged controls. Birds fed antimicrobials were protected from NE development as indicated by maintenance of body weight, low mortality and clostridium levels, and decreased intestinal macroscopic lesion scores compared to challenged controls (P < 0·05). 'Lactobacillus johnsonii'-fed birds had reduced lesion scores, whilst OA-fed birds had decreased 'Cl. perfringens' levels. Both 'Lact. johnsonii' and OA-fed birds had improved feed efficiency between days 0 and 28 compared to challenged controls; however, mortality and body weights were not improved by either treatment. Microbial profiling indicated that the challenge procedure significantly altered the jejunal microbiota. The microbiota of antimicrobial-fed birds was significantly different from all other groups. Conclusions: Whilst 'Lact. johnsonii' and OA altered specific intestinal parameters, significant protection against NE was not observed. Significance and Impact of the Study: 'Lactobacillus johnsonii' and OA did not prevent NE; however, some improvements were evident. Other related treatments, or combinations of these two treatments, may provide greater protection.
Citation	Journal of Applied Microbiology, 109(4), p. 1329-1338
ISSN	1365-2672 1364-5072
Link	https://hdl.handle.net/1959.11/7758
Language	en
Publisher	Wiley-Blackwell Publishing Ltd
Title	Comparison of alternatives to in-feed antimicrobials for the prevention of clinical necrotic enteritis
Type of document	Journal Article
Entity Type	Publication

Comparison of alternatives to in-feed antimicrobials for the prevention of clinical necrotic enteritis

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