| Author(s) |
Wu, Shubiao
Rodgers, Nicholas
Choct, Mingan
|
| Publication Date |
2011
|
| Abstract |
We compared ileal 'Clostridium perfringens' quantification results produced by real-time PCR and culture-based methods in broiler chickens in a challenge model of necrotic enteritis. Assessment of the relative standard deviations (RSDs) revealed that the real-time PCR assay generated a smaller standard deviation and thus was more precise than the culture-based method. Linear regression analysis indicated that the bacterial counts of these two methods were highly correlated (R2 = 0.845). We suggest that real-time PCR could be a replacement of the culture method for quantifying 'C. perfringens' in the intestinal tracts of broiler chickens.
|
| Citation |
Applied and Environmental Microbiology, 77(3), p. 1135-1139
|
| ISSN |
1098-5336
0099-2240
|
| Link | |
| Language |
en
|
| Publisher |
American Society for Microbiology
|
| Title |
Real-Time PCR Assay for 'Clostridium perfringens' in Broiler Chickens in a Challenge Model of Necrotic Enteritis
|
| Type of document |
Journal Article
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| Entity Type |
Publication
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