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|Title:||Real-Time PCR Assay for 'Clostridium perfringens' in Broiler Chickens in a Challenge Model of Necrotic Enteritis||Contributor(s):||Wu, Shubiao (author) ; Rodgers, Nicholas (author); Choct, Mingan (author)||Publication Date:||2011||Open Access:||Yes||DOI:||10.1128/AEM.01803-10||Handle Link:||https://hdl.handle.net/1959.11/7177||Abstract:||We compared ileal 'Clostridium perfringens' quantification results produced by real-time PCR and culture-based methods in broiler chickens in a challenge model of necrotic enteritis. Assessment of the relative standard deviations (RSDs) revealed that the real-time PCR assay generated a smaller standard deviation and thus was more precise than the culture-based method. Linear regression analysis indicated that the bacterial counts of these two methods were highly correlated (R2 = 0.845). We suggest that real-time PCR could be a replacement of the culture method for quantifying 'C. perfringens' in the intestinal tracts of broiler chickens.||Publication Type:||Journal Article||Source of Publication:||Applied and Environmental Microbiology, 77(3), p. 1135-1139||Publisher:||American Society for Microbiology||Place of Publication:||United States of America||ISSN:||1098-5336
|Field of Research (FOR):||070205 Animal Protection (Pests and Pathogens)||Peer Reviewed:||Yes||HERDC Category Description:||C1 Refereed Article in a Scholarly Journal||Statistics to Oct 2018:||Visitors: 480
|Appears in Collections:||Journal Article|
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