Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/7177
Title: Real-Time PCR Assay for 'Clostridium perfringens' in Broiler Chickens in a Challenge Model of Necrotic Enteritis
Contributor(s): Wu, Shubiao  (author)orcid ; Rodgers, Nicholas  (author); Choct, Mingan  (author)orcid 
Publication Date: 2011
Open Access: Yes
DOI: 10.1128/AEM.01803-10Open Access Link
Handle Link: https://hdl.handle.net/1959.11/7177
Abstract: We compared ileal 'Clostridium perfringens' quantification results produced by real-time PCR and culture-based methods in broiler chickens in a challenge model of necrotic enteritis. Assessment of the relative standard deviations (RSDs) revealed that the real-time PCR assay generated a smaller standard deviation and thus was more precise than the culture-based method. Linear regression analysis indicated that the bacterial counts of these two methods were highly correlated (R2 = 0.845). We suggest that real-time PCR could be a replacement of the culture method for quantifying 'C. perfringens' in the intestinal tracts of broiler chickens.
Publication Type: Journal Article
Source of Publication: Applied and Environmental Microbiology, 77(3), p. 1135-1139
Publisher: American Society for Microbiology
Place of Publication: United States of America
ISSN: 1098-5336
0099-2240
Field of Research (FOR): 070205 Animal Protection (Pests and Pathogens)
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Statistics to Oct 2018: Visitors: 480
Views: 490
Downloads: 0
Appears in Collections:Journal Article

Files in This Item:
3 files
File Description SizeFormat 
Show full item record

SCOPUSTM   
Citations

18
checked on Nov 30, 2018

Page view(s)

116
checked on Feb 8, 2019
Google Media

Google ScholarTM

Check

Altmetric


Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.