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Title: Real-Time PCR Assay for 'Clostridium perfringens' in Broiler Chickens in a Challenge Model of Necrotic Enteritis
Contributor(s): Wu, Shubiao  (author)orcid ; Rodgers, Nicholas  (author); Choct, Mingan  (author)orcid 
Publication Date: 2011
Open Access: Yes
DOI: 10.1128/AEM.01803-10Open Access Link
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Abstract: We compared ileal 'Clostridium perfringens' quantification results produced by real-time PCR and culture-based methods in broiler chickens in a challenge model of necrotic enteritis. Assessment of the relative standard deviations (RSDs) revealed that the real-time PCR assay generated a smaller standard deviation and thus was more precise than the culture-based method. Linear regression analysis indicated that the bacterial counts of these two methods were highly correlated (R2 = 0.845). We suggest that real-time PCR could be a replacement of the culture method for quantifying 'C. perfringens' in the intestinal tracts of broiler chickens.
Publication Type: Journal Article
Source of Publication: Applied and Environmental Microbiology, 77(3), p. 1135-1139
Publisher: American Society for Microbiology
Place of Publication: United States of America
ISSN: 1098-5336
Field of Research (FOR): 070205 Animal Protection (Pests and Pathogens)
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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