Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/6666
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dc.contributor.authorCiller, U Aen
dc.contributor.authorCiller, I Men
dc.contributor.authorMcFarlane, J Ren
dc.date.accessioned2010-10-07T15:07:00Z-
dc.date.issued2008-
dc.identifier.citationReproduction, Fertility and Development, v.20 SRB Abstracts (Supplement), p. 77-77en
dc.identifier.issn1448-5990en
dc.identifier.issn1031-3613en
dc.identifier.urihttps://hdl.handle.net/1959.11/6666-
dc.description.abstractIt is well documented that there is considerable batch to batch variation in the activity of commercial preparations of gonadotrophins. These products are used in a variety of assisted reproductive procedures in the livestock industry, consequently this high degree of variation between products and batches adds to the already considerable between animal variations in response to the treatment. Equine chorionic gonadotrophin (eCG) is a heterodimeric glycoprotein hormone secreted by the placental endometrial cups during the first third of gestation in the horse. Plasma is harvested from pregnant mares between 40–90 days of gestation and the eCG isolated and used to formulate commercial preparations. Previous research has shown that eCG like the other gonadotrophins is a highly heterogeneous molecule with significant differences in bioactivity between isoforms. The aim of this study was to determine whether significant differences in isoform composition exist between various commercial preparations of eCG (n = 15), and how this compares with the isoform composition found in plasma (n = 23). Concentrations of eCG were determined using a competitive eCG ELISA. Liquid phase iso-electric focusing was used to fractionate plasma and the commercial preparations into 10 pH ranges from pH 3.0 to pH 10.0. Data from the 10 fractions were grouped into acidic (pH 3.0–5.1), intermediate (pH 5.2–7.9), or basic (pH 8.0–10.0) isoform categories for analysis. Immunoactivity between commercial eCG products ranged from 44% to 362% of stated bioactivity. Iso-electric focusing showed that the majority of the immunoactivity (92%) of the commercial preparations was found in the acidic fractions (pH 3.0–5.1), and in particular in the pH range 3.0–3.8. This contrasted starkly with isoform profiles found in pregnant mare plasma samples which showed a much greater spread across all 3 pH ranges. In summary, the isolation processes of commercial eCG preparations appears to selectively favour the acidic isoforms of eCG.en
dc.languageenen
dc.publisherCSIRO Publishingen
dc.relation.ispartofReproduction, Fertility and Developmenten
dc.titleEquine chorionic gonadotrophin isoform composition in commercial products compared to isoform composition in pregnant mare plasmaen
dc.typeConference Publicationen
dc.relation.conferenceSRB 2008: 39th Annual Conference of the Society for Reproductive Biologyen
dc.subject.keywordsAnimal Physiology - Systemsen
local.contributor.firstnameU Aen
local.contributor.firstnameI Men
local.contributor.firstnameJ Ren
local.subject.for2008060603 Animal Physiology - Systemsen
local.subject.seo2008970111 Expanding Knowledge in the Medical and Health Sciencesen
local.profile.schoolSchool of Science and Technologyen
local.profile.schoolSchool of Science and Technologyen
local.profile.schoolSchool of Science and Technologyen
local.profile.emailuciller2@une.edu.auen
local.profile.emailiciller2@une.edu.auen
local.profile.emailjmcfarla@une.edu.auen
local.output.categoryE3en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20100422-132144en
local.date.conference25th - 28th August, 2008en
local.conference.placeMelbourne, Australiaen
local.publisher.placeAustraliaen
local.format.startpage77en
local.format.endpage77en
local.identifier.volume20 SRB Abstracts (Supplement)en
local.contributor.lastnameCilleren
local.contributor.lastnameCilleren
local.contributor.lastnameMcFarlaneen
dc.identifier.staffune-id:uciller2en
dc.identifier.staffune-id:iciller2en
dc.identifier.staffune-id:jmcfarlaen
local.profile.orcid0000-0003-4429-5384en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:6826en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleEquine chorionic gonadotrophin isoform composition in commercial products compared to isoform composition in pregnant mare plasmaen
local.output.categorydescriptionE3 Extract of Scholarly Conference Publicationen
local.relation.urlhttp://www.publish.csiro.au/paper/SRB08Abs277en
local.relation.urlhttp://www.publish.csiro.au/nid/44/issue/4038.htmen
local.conference.detailsSRB 2008: 39th Annual Conference of the Society for Reproductive Biology, Melbourne, Australia, 25th - 28th August, 2008en
local.search.authorCiller, U Aen
local.search.authorCiller, I Men
local.search.authorMcFarlane, J Ren
local.uneassociationUnknownen
local.year.published2008en
local.date.start2008-08-25-
local.date.end2008-08-28-
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School of Science and Technology
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