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Title: Analysis of cotton ('Gossypium hirsutum') root proteomes during a compatible interaction with the black root rot fungus 'Thielaviopsis basicola'
Contributor(s): Coumans-Moens, Joelle  (author)orcid ; Poljak, Anne (author); Raftery, Mark J (author); Backhouse, David  (author)orcid ; Pereg, Lily  (author)
Publication Date: 2009
DOI: 10.1002/pmic.200800251
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Abstract: A proteomic approach was used to uncover the inducible molecular defense mechanism of cotton root occurring during the compatible interaction with 'Thielaviopsis basicola'. Microscopic observation of cotton root inoculated with a suspension of conidia showed that this necrotrophic hemibiotroph fungus interacts with the plant and completes its life cycle in our experimental system. 2-DE analysis of root extracts taken after 1, 3, 5, and 7 days postinoculation and cluster analysis of the protein expression levels showed four major profiles (constant, upregulated, one slightly downregulated, and one dramatically downregulated). Spots significantly (p<0.05) upregulated were analyzed by LC-MS/MS and identified using MASCOT MS/MS ion search software and associated databases. These proteins included defense and stress related proteins, such as pathogenesis-related proteins and proteins likely to be involved in the oxidative burst, sugar, and nitrogen metabolism as well as amino acid and isoprenoid synthesis. While many of the identified proteins are common components of the defense response of most plants, a proteasome subunit and a protein reported to be induced only in cotton root following 'Meloidogyne incognita' infection were also identified.
Publication Type: Journal Article
Source of Publication: Proteomics, 9(2), p. 335-349
Publisher: Wiley-VCH Verlag GmbH & Co KGaA
Place of Publication: Weinheim, Germany
ISSN: 1615-9861
Field of Research (FOR): 060799 Plant Biology not elsewhere classified
060109 Proteomics and Intermolecular Interactions (excl Medical Proteomics)
Socio-Economic Objective (SEO): 829999 Plant Production and Plant Primary Products not elsewhere classified
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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