Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/62752
Title: Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)
Contributor(s): Cossarizza, Andrea (author); Chang, Hyun-Dong (author); Radbruch, Andreas (author); Acs, Andreas (author); Adam, Dieter (author); Adam-Klages, Sabine (author); Agace, William W (author); Aghaeepour, Nima (author); Akdis, Mübeccel (author); Allez, Matthieu (author); Nogueira Almeida, Larissa (author); Alvisi, Giorgia (author); Anderson, Graham (author); Andrä, Immanuel (author); Annunziato, Francesco (author); Anselmo, Achille (author); Bacher, Petra (author); Baldari, Cosima T (author); Bari, Sudipto (author); Barnaba, Vincenzo (author); Barros-Martins, Joana (author); Battistini, Luca (author); Bauer, Wolfgang (author); Baumgart, Sabine (author); Baumgarth, Nicole (author); Baumjohann, Dirk (author); Baying, Bianka (author); Bebawy, Mary  (author)orcid ; Becher, Burkhard (author); Beisker, Wolfgang (author); Benes, Vladimir (author); Beyaert, Rudi (author); Blanco, Alfonso (author); Boardman, Dominic A (author); Bogdan, Christian (author); Borger, Jessica G (author); Borsellino, Giovanna (author); Boulais, Philip E (author); Bradford, Jolene A (author); Brenner, Dirk (author); Brinkman, Ryan R (author); Brooks, Anna E S (author); Busch, Dirk H (author); Büscher, Martin (author); Bushnell, Timothy P (author); Calzetti, Federica (author); Cameron, Garth (author); Cammarata, Ilenia (author); Cao, Xuetao (author); Cardell, Susanna L (author); Casola, Stefano (author); Cassatella, Marco A (author); Cavani, Andrea (author); Celada, Antonio (author); Chatenoud, Lucienne (author); Chattopadhyay, Pratip K (author); Chow, Sue (author); Christakou, Eleni (author); Čičin-Šain, Luka (author); Clerici, Mario (author); Colombo, Federico S (author); Cook, Laura (author); Cooke, Anne (author); Cooper, Andrea M (author); Corbett, Alexandra J (author); Cosma, Antonio (author); Cosmi, Lorenzo (author); Coulie, Pierre G (author); Cumano, Ana (author); Cvetkovic, Ljiljana (author); Dang, Van Duc (author); Dang-Heine, Chantip (author); S Davey, Martin (author); Davies, Derek (author); Biasi, Sara De (author); Del Zotto, Genny (author); Victoriano Dela Cruz, Gelo (author); Delacher, Michael (author); Della Bella, Silvia (author); Dellabona, Paolo (author); Deniz, Günnur (author); Dessing, Mark (author); Santo, James P Di (author); Diefenbach, Andreas (author); Dieli, Francesco (author); Dolf, Andreas (author); Dörner, Thomas (author); Dress, Regine J (author); Dudziak, Diana (author); Dustin, Michael (author); Dutertre, Charles-Antoine (author); Ebner, Friederike (author); Eckle, Sidonia B G (author); Edinger, Matthias (author); Eede, Pascale (author); Ehrhardt, Götz R A (author); Eich, Marcus (author); Engel, Pablo (author); Engelhardt, Britta (author); Erdei, Anna (author); Esser, Charlotte (author); Everts, Bart (author); Evrard, Maximilien (author); Falk, Christine S (author); Fehniger, Todd A (author); Felipo-Benavent, Mar (author); Ferry, Helen (author); Feuerer, Markus (author); Filby, Andrew (author); Filkor, Kata (author); Fillatreau, Simon (author); Follo, Marie (author); Förster, Irmgard (author); Foster, John (author); Foulds, Gemma A (author); Frehse, Britta (author); Frenette, Paul S (author); Frischbutter, Stefan (author); Fritzsche, Wolfgang (author); Galbraith, David W (author); Gangaev, Anastasia (author); Garbi, Natalio (author); Gaudilliere, Brice (author); Gazzinelli, Ricardo T (author); Geginat, Jens (author); Gerner, Wilhelm (author); Gherardin, Nicholas A (author); Ghoreschi, Kamran (author); Gibellini, Lara (author); Ginhoux, Florent (author); Goda, Keisuke (author); Godfrey, Dale I (author); Goettlinger, Christoph (author); González-Navajas, Jose M (author); Goodyear, Carl S (author); Gori, Andrea (author); Grogan, Jane L (author); Grummitt, Daryl (author); Grützkau, Andreas (author); Haftmann, Claudia (author); Hahn, Jonas (author); Hammad, Hamida (author); Hämmerling, Günter (author); Hansmann, Leo (author); Hansson, Goran (author); Harpur, Christopher M (author); Hartmann, Susanne (author); Hauser, Andrea (author); Hauser, Anja E (author); Haviland, David L (author); Hedley, David (author); Hernández, Daniela C (author); Herrera, Guadalupe (author); Herrmann, Martin (author); Hess, Christoph (author); Höfer, Thomas (author); Hoffmann, Petra (author); Hogquist, Kristin (author); Holland, Tristan (author); Höllt, Tristan (author); Holmdahl, Rikard (author); Hombrink, Pleun (author); Houston, Jessica P (author); Hoyer, Bimba F (author); Huang, Bo (author); Huang, Fang-Ping (author); Huber, Johanna E (author); Huehn, Jochen (author); Hundemer, Michael (author); Hunter, Christopher A (author); Hwang, William Y K (author); Iannone, Anna (author); Ingelfinger, Florian (author); Ivison, Sabine M (author); Jäck, Hans-Martin (author); Jani, Peter K (author); Jávega, Beatriz (author); Jonjic, Stipan (author); Kaiser, Toralf (author); Kalina, Tomas (author); Kamradt, Thomas (author); Kaufmann, Stefan H E (author); Keller, Baerbel (author); Ketelaars, Steven L C (author); Khalilnezhad, Ahad (author); Khan, Srijit (author); Kisielow, Jan (author); Klenerman, Paul (author); Knopf, Jasmin (author); Koay, Hui-Fern (author); Kobow, Katja (author); Kolls, Jay K (author); Kong, Wan Ting (author); Kopf, Manfred (author); Korn, Manfred (author); Kriegsmann, Katharina (author); Kristyanto, Hendy (author); Kroneis, Thomas (author); Krueger, Andreas (author); Kühne, Jenny (author); Kukat, Christian (author); Kunkel, Désirée (author); Kunze-Schumacher, Heike (author); Kurosaki, Tomohiro (author); Kurts, Christian (author); Kvistborg, Pia (author); Kwok, Immanuel (author); Landry, Jonathan (author); Lantz, Olivier (author); Lanuti, Paola (author); LaRosa, Francesca (author); Lehuen, Agnès (author); LeibundGut-Landmann, Salomé (author); Leipold, Michael D (author); Leung, Leslie Y T (author); Levings, Megan K (author); Lino, Andreia C (author); Liotta, Francesco (author); Litwin, Virginia (author); Liu, Yanling (author); Ljunggren, Hans-Gustaf (author); Lohoff, Michael (author); Lombardi, Giovanna (author); Lopez, Lilly (author); López-Botet, Miguel (author); E Lovett-Racke, Amy (author); Lubberts, Erik (author); Luche, Herve (author); Ludewig, Burkhard (author); Lugli, Enrico (author); Lunemann, Sebastian (author); Maecker, Holden T (author); Maggi, Laura (author); Maguire, Orla (author); Mair, Florian (author); Mair, Kerstin H (author); Mantovani, Alberto (author); Manz, Rudolf A (author); Marshall, Aaron J (author); Martínez-Romero, Alicia (author); Martrus, Glòria (author); Marventano, Ivana (author); Maslinski, Wlodzimierz (author); Matarese, Giuseppe (author); Mattioli, Anna Vittoria (author); Maueröder, Christian (author); Mazzoni, Alessio (author); McCluskey, James (author); McGrath, Mairi (author); McGuire, Helen M (author); McInnes, Iain B (author); Mei, Henrik E (author); Melchers, Fritz (author); Melzer, Susanne (author); Mielenz, Dirk (author); Miller, Stephen D (author); Mills, Kingston H G (author); Minderman, Hans (author); Mjösberg, Jenny (author); Moore, Jonni (author); Moran, Barry (author); Moretta, Lorenzo (author); Mosmann, Tim R (author); Müller, Susann (author); Multhoff, Gabriele (author); Muñoz, Luis Enrique (author); Münz, Christian (author); Nakayama, Toshinori (author); Nasi, Milena (author); Neumann, Katrin (author); Ng, Lai Guan (author); Niedobitek, Antonia (author); Nourshargh, Sussan (author); Núñez, Gabriel (author); O'Connor, José-Enrique (author); Ochel, Aaron (author); Oja, Anna (author); Ordonez, Diana (author); Orfao, Diana (author); Orlowski-Oliver, Eva (author); Ouyang, Wenjun (author); Oxenius, Annette (author); Palankar, Raghavendra (author); Panse, Isabel (author); Pattanapanyasat, Kovit (author); Paulsen, Malte (author); Pavlinic, Dinko (author); Penter, Livius (author); Peterson, Pärt (author); Peth, Christian (creator); Petriz, Jordi (author); Piancone, Federica (author); Pickl, Winfried F (author); Piconese, Silvia (author); Pinti, Marcello (author); Pockley, A Graham (author); Podolska, Malgorzata Justyna (author); Poon, Zhiyong (author); Pracht, Katharina (author); Prinz, Immo (author); Pucillo, Carlo E M (author); Quataert, Sally A (author); Quatrini, Linda (author); Quinn, Kylie M (author); Radbruch, Helena (author); Radstake, Tim R D J (author); Rahmig, Susann (author); Rahn, Hans-Peter (author); Rajwa, Bartek (author); Ravichandran, Gevitha (author); Raz, Yotam (author); Rebhahn, Jonathan A (author); Recktenwald, Diether (author); Reimer, Dorothea (author); Reis e Sousa, Caetano (author); Remmerswaal, Ester B M (author); Richter, Lisa (author); Rico, Laura G (author); Riddell, Andy (author); Rieger, Aja M (author); Robinson, J Paul (author); Romagnani, Chiara (author); Rubartelli, Anna (author); Ruland, Jürgen (author); Saalmüller, Armin (author); Saeys, Yvan (author); Saito, Takashi (author); Sakaguchi, Shimon (author); Sala-de-Oyanguren, Francisco (author); Samstag, Yvonne (author); Sanderson, Sharon (author); Sandrock, Inga (author); Santoni, Angela (author); Sanz, Ramon Bellmàs (author); Saresella, Marina (author); Sautes-Fridman, Catherine (author); Sawitzki, Birgit (author); Schadt, Linda (author); Scheffold, Alexander (author); Scherer, Hans U (author); Schiemann, Matthias (author); Schildberg, Frank A (author); Schimisky, Esther (author); Schlitzer, Andreas (author); Schlosser, Josephine (author); Schmid, Stephan (author); Schmitt, Steffen (author); Schober, Kilian (author); Schraivogel, Daniel (author); Schuh, Wolfgang (author); Schüler, Thomas (author); Schulte, Reiner (author); Ronald Schulz, Axel (author); R Schulz, Sebastian (author); Scottá, Cristiano (author); Scott-Algara, Daniel (author); Sester, David P (author); Shankey, T Vincent (author); Silva-Santos, Bruno (author); Katharina Simon, Anna (author); Sitnik, Katarzyna M (author); Sozzani, Silvano (author); Speiser, Daniel E (author); Spidlen, Josef (author); Stahlberg, Anders (author); Stall, Alan M (author); Stanley, Natalie (author); Stark, Regina (author); Stehle, Christina (author); Steinmetz, Tobit (author); Stockinger, Hannes (author); Takahama, Yousuke (author); Takeda, Kiyoshi (author); Tan, Leonard (author); Tárnok, Attila (author); Tiegs, Gisa (author); Toldi, Gergely (author); Tornack, Julia (author); Traggiai, Elisabetta (author); Trebak, Mohamed (author); Tree, Timothy I M (author); Trotter, Joe (author); Trowsdale, John (author); Tsoumakidou, Maria (author); Ulrich, Henning (author); Urbanczyk, Sophia (author); van de Veen, Willem (author); van den Broek, Maries (author); van der Pol, Edwin (author); Gassen, Sofie Van (author); Isterdael, Gert Van (author); A W van Lier, René (author); Veldhoen, Marc (author); Vento-Asturias, Salvador (author); Vieira, Paulo (author); Voehringer, David (author); Volk, Hans-Dieter (author); von Borstel, Anouk (author); von Volkmann, Konrad (author); Waisman, Ari (author); Walker, Rachael V (author); Wallace, Paul K (author); Wang, Sa A (author); Wang, Xin M (author); Ward, Michael D (author); Ward-Hartstonge, Kirsten A (author); Warnatz, Klaus (author); Warnes, Gary (author); Warth, Sarah (author); Waskow, Claudia (author); Watson, James V (author); Watzl, Carsten (author); Wegener, Leonie (author); Weisenburger, Thomas (author); Wiedemann, Annika (author); Wienands, Jürgen (author); Wilharm, Anneke (author); Wilkinson, Robert John (author); Willimsky, Gerald (author); Wing, James B (author); Winkelmann, Rieke (author); Winkler, Thomas H (author); Wirz, Oliver F (author); Wong, Alicia (author); Wurst, Peter (author); Yang, Jennie H M (author); Yang, Juhao (author); Yazdanbakhsh, Maria (author); Yu, Liping (author); Yue, Alice (author); Zhang, Hanlin (author); Zhao, Yi (author); Ziegler, Susanne Maria (author); Zielinski, Christina (author); Zimmermann, Jakob (author); Zychlinsky, Arturo (author)
Publication Date: 2019
DOI: 10.1002/eji.201970107
Handle Link: https://hdl.handle.net/1959.11/62752
Abstract: 

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all writ-ten and peer-reviewed by leading experts in the field, making this an essential research companion.

Publication Type: Journal Article
Source of Publication: Eur J Immunol, v.49 (10), p. 1457-1973
Fields of Research (FoR) 2020: 3208 Medical physiology
Socio-Economic Objective (SEO) 2020: TBD
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Publisher/associated links: https://app.dimensions.ai/details/publication/pub.1121951380
Appears in Collections:Journal Article

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