Guidelines for the use of flow cytometry and cell sorting in immunological studies (second edition)

2019

Cossarizza, Andrea

Chang, Hyun-Dong

Radbruch, Andreas

Acs, Andreas

Adam, Dieter

Adam-Klages, Sabine

Agace, William W

Aghaeepour, Nima

Akdis, Mübeccel

Allez, Matthieu

Nogueira Almeida, Larissa

Alvisi, Giorgia

Anderson, Graham

Andrä, Immanuel

Annunziato, Francesco

Anselmo, Achille

Bacher, Petra

Baldari, Cosima T

Bari, Sudipto

Barnaba, Vincenzo

Barros-Martins, Joana

Battistini, Luca

Bauer, Wolfgang

Baumgart, Sabine

Baumgarth, Nicole

Baumjohann, Dirk

Baying, Bianka

Bebawy, Mary

Becher, Burkhard

Beisker, Wolfgang

Benes, Vladimir

Beyaert, Rudi

Blanco, Alfonso

Boardman, Dominic A

Bogdan, Christian

Borger, Jessica G

Borsellino, Giovanna

Boulais, Philip E

Bradford, Jolene A

Brenner, Dirk

Brinkman, Ryan R

Brooks, Anna E S

Busch, Dirk H

Büscher, Martin

Bushnell, Timothy P

Calzetti, Federica

Cameron, Garth

Cammarata, Ilenia

Cao, Xuetao

Cardell, Susanna L

Casola, Stefano

Cassatella, Marco A

Cavani, Andrea

Celada, Antonio

Chatenoud, Lucienne

Chattopadhyay, Pratip K

Chow, Sue

Christakou, Eleni

Čičin-Šain, Luka

Clerici, Mario

Colombo, Federico S

Cook, Laura

Cooke, Anne

Cooper, Andrea M

Corbett, Alexandra J

Cosma, Antonio

Cosmi, Lorenzo

Coulie, Pierre G

Cumano, Ana

Cvetkovic, Ljiljana

Dang, Van Duc

Dang-Heine, Chantip

S Davey, Martin

Davies, Derek

Biasi, Sara De

Del Zotto, Genny

Victoriano Dela Cruz, Gelo

Delacher, Michael

Della Bella, Silvia

Dellabona, Paolo

Deniz, Günnur

Dessing, Mark

Santo, James P Di

Diefenbach, Andreas

Dieli, Francesco

Dolf, Andreas

Dörner, Thomas

Dress, Regine J

Dudziak, Diana

Dustin, Michael

Dutertre, Charles-Antoine

Ebner, Friederike

Eckle, Sidonia B G

Edinger, Matthias

Eede, Pascale

Ehrhardt, Götz R A

Eich, Marcus

Engel, Pablo

Engelhardt, Britta

Erdei, Anna

Esser, Charlotte

Everts, Bart

Evrard, Maximilien

Falk, Christine S

Fehniger, Todd A

Felipo-Benavent, Mar

Ferry, Helen

Feuerer, Markus

Filby, Andrew

Filkor, Kata

Fillatreau, Simon

Follo, Marie

Förster, Irmgard

Foster, John

Foulds, Gemma A

Frehse, Britta

Frenette, Paul S

Frischbutter, Stefan

Fritzsche, Wolfgang

Galbraith, David W

Gangaev, Anastasia

Garbi, Natalio

Gaudilliere, Brice

Gazzinelli, Ricardo T

Geginat, Jens

Gerner, Wilhelm

Gherardin, Nicholas A

Ghoreschi, Kamran

Gibellini, Lara

Ginhoux, Florent

Goda, Keisuke

Godfrey, Dale I

Goettlinger, Christoph

González-Navajas, Jose M

Goodyear, Carl S

Gori, Andrea

Grogan, Jane L

Grummitt, Daryl

Grützkau, Andreas

Haftmann, Claudia

Hahn, Jonas

Hammad, Hamida

Hämmerling, Günter

Hansmann, Leo

Hansson, Goran

Harpur, Christopher M

Hartmann, Susanne

Hauser, Andrea

Hauser, Anja E

Haviland, David L

Hedley, David

Hernández, Daniela C

Herrera, Guadalupe

Herrmann, Martin

Hess, Christoph

Höfer, Thomas

Hoffmann, Petra

Hogquist, Kristin

Holland, Tristan

Höllt, Tristan

Holmdahl, Rikard

Hombrink, Pleun

Houston, Jessica P

Hoyer, Bimba F

Huang, Bo

Huang, Fang-Ping

Huber, Johanna E

Huehn, Jochen

Hundemer, Michael

Hunter, Christopher A

Hwang, William Y K

Iannone, Anna

Ingelfinger, Florian

Ivison, Sabine M

Jäck, Hans-Martin

Jani, Peter K

Jávega, Beatriz

Jonjic, Stipan

Kaiser, Toralf

Kalina, Tomas

Kamradt, Thomas

Kaufmann, Stefan H E

Keller, Baerbel

Ketelaars, Steven L C

Khalilnezhad, Ahad

Khan, Srijit

Kisielow, Jan

Klenerman, Paul

Knopf, Jasmin

Koay, Hui-Fern

Kobow, Katja

Kolls, Jay K

Kong, Wan Ting

Kopf, Manfred

Korn, Manfred

Kriegsmann, Katharina

Kristyanto, Hendy

Kroneis, Thomas

Krueger, Andreas

Kühne, Jenny

Kukat, Christian

Kunkel, Désirée

Kunze-Schumacher, Heike

Kurosaki, Tomohiro

Kurts, Christian

Kvistborg, Pia

Kwok, Immanuel

Landry, Jonathan

Lantz, Olivier

Lanuti, Paola

LaRosa, Francesca

Lehuen, Agnès

LeibundGut-Landmann, Salomé

Leipold, Michael D

Leung, Leslie Y T

Levings, Megan K

Lino, Andreia C

Liotta, Francesco

Litwin, Virginia

Liu, Yanling

Ljunggren, Hans-Gustaf

Lohoff, Michael

Lombardi, Giovanna

Lopez, Lilly

López-Botet, Miguel

E Lovett-Racke, Amy

Lubberts, Erik

Luche, Herve

Ludewig, Burkhard

Lugli, Enrico

Lunemann, Sebastian

Maecker, Holden T

Maggi, Laura

Maguire, Orla

Mair, Florian

Mair, Kerstin H

Mantovani, Alberto

Manz, Rudolf A

Marshall, Aaron J

Martínez-Romero, Alicia

Martrus, Glòria

Marventano, Ivana

Maslinski, Wlodzimierz

Matarese, Giuseppe

Mattioli, Anna Vittoria

Maueröder, Christian

Mazzoni, Alessio

McCluskey, James

McGrath, Mairi

McGuire, Helen M

McInnes, Iain B

Mei, Henrik E

Melchers, Fritz

Melzer, Susanne

Mielenz, Dirk

Miller, Stephen D

Mills, Kingston H G

Minderman, Hans

Mjösberg, Jenny

Moore, Jonni

Moran, Barry

Moretta, Lorenzo

Mosmann, Tim R

Müller, Susann

Multhoff, Gabriele

Muñoz, Luis Enrique

Münz, Christian

Nakayama, Toshinori

Nasi, Milena

Neumann, Katrin

Ng, Lai Guan

Niedobitek, Antonia

Nourshargh, Sussan

Núñez, Gabriel

O'Connor, José-Enrique

Ochel, Aaron

Oja, Anna

Ordonez, Diana

Orfao, Diana

Orlowski-Oliver, Eva

Ouyang, Wenjun

Oxenius, Annette

Palankar, Raghavendra

Panse, Isabel

Pattanapanyasat, Kovit

Paulsen, Malte

Pavlinic, Dinko

Penter, Livius

Peterson, Pärt

Peth, Christian

Petriz, Jordi

Piancone, Federica

Pickl, Winfried F

Piconese, Silvia

Pinti, Marcello

Pockley, A Graham

Podolska, Malgorzata Justyna

Poon, Zhiyong

Pracht, Katharina

Prinz, Immo

Pucillo, Carlo E M

Quataert, Sally A

Quatrini, Linda

Quinn, Kylie M

Radbruch, Helena

Radstake, Tim R D J

Rahmig, Susann

Rahn, Hans-Peter

Rajwa, Bartek

Ravichandran, Gevitha

Raz, Yotam

Rebhahn, Jonathan A

Recktenwald, Diether

Reimer, Dorothea

Reis e Sousa, Caetano

Remmerswaal, Ester B M

Richter, Lisa

Rico, Laura G

Riddell, Andy

Rieger, Aja M

Robinson, J Paul

Romagnani, Chiara

Rubartelli, Anna

Ruland, Jürgen

Saalmüller, Armin

Saeys, Yvan

Saito, Takashi

Sakaguchi, Shimon

Sala-de-Oyanguren, Francisco

Samstag, Yvonne

Sanderson, Sharon

Sandrock, Inga

Santoni, Angela

Sanz, Ramon Bellmàs

Saresella, Marina

Sautes-Fridman, Catherine

Sawitzki, Birgit

Schadt, Linda

Scheffold, Alexander

Scherer, Hans U

Schiemann, Matthias

Schildberg, Frank A

Schimisky, Esther

Schlitzer, Andreas

Schlosser, Josephine

Schmid, Stephan

Schmitt, Steffen

Schober, Kilian

Schraivogel, Daniel

Schuh, Wolfgang

Schüler, Thomas

Schulte, Reiner

Ronald Schulz, Axel

R Schulz, Sebastian

Scottá, Cristiano

Scott-Algara, Daniel

Sester, David P

Shankey, T Vincent

Silva-Santos, Bruno

Katharina Simon, Anna

Sitnik, Katarzyna M

Sozzani, Silvano

Speiser, Daniel E

Spidlen, Josef

Stahlberg, Anders

Stall, Alan M

Stanley, Natalie

Stark, Regina

Stehle, Christina

Steinmetz, Tobit

Stockinger, Hannes

Takahama, Yousuke

Takeda, Kiyoshi

Tan, Leonard

Tárnok, Attila

Tiegs, Gisa

Toldi, Gergely

Tornack, Julia

Traggiai, Elisabetta

Trebak, Mohamed

Tree, Timothy I M

Trotter, Joe

Trowsdale, John

Tsoumakidou, Maria

Ulrich, Henning

Urbanczyk, Sophia

van de Veen, Willem

van den Broek, Maries

van der Pol, Edwin

Gassen, Sofie Van

Isterdael, Gert Van

A W van Lier, René

Veldhoen, Marc

Vento-Asturias, Salvador

Vieira, Paulo

Voehringer, David

Volk, Hans-Dieter

von Borstel, Anouk

von Volkmann, Konrad

Waisman, Ari

Walker, Rachael V

Wallace, Paul K

Wang, Sa A

Wang, Xin M

Ward, Michael D

Ward-Hartstonge, Kirsten A

Warnatz, Klaus

Warnes, Gary

Warth, Sarah

Waskow, Claudia

Watson, James V

Watzl, Carsten

Wegener, Leonie

Weisenburger, Thomas

Wiedemann, Annika

Wienands, Jürgen

Wilharm, Anneke

Wilkinson, Robert John

Willimsky, Gerald

Wing, James B

Winkelmann, Rieke

Winkler, Thomas H

Wirz, Oliver F

Wong, Alicia

Wurst, Peter

Yang, Jennie H M

Yang, Juhao

Yazdanbakhsh, Maria

Yu, Liping

Yue, Alice

Zhang, Hanlin

Zhao, Yi

Ziegler, Susanne Maria

Zielinski, Christina

Zimmermann, Jakob

Zychlinsky, Arturo

Journal Article

en

Publication

10.1002/eji.201970107

une:1959.11/62752

These guidelines are a consensus work of a considerable number of members of the immunology and flow cytometry community. They provide the theory and key practical aspects of flow cytometry enabling immunologists to avoid the common errors that often undermine immunological data. Notably, there are comprehensive sections of all major immune cell types with helpful Tables detailing phenotypes in murine and human cells. The latest flow cytometry techniques and applications are also described, featuring examples of the data that can be generated and, importantly, how the data can be analysed. Furthermore, there are sections detailing tips, tricks and pitfalls to avoid, all writ-ten and peer-reviewed by leading experts in the field, making this an essential research companion.

link

Eur J Immunol, v.49 (10), p. 1457-1973

1457-1973