Plasma lipopolysaccharide elevations in cattle associated with early-stage infection by Fasciola hepatica

Author(s)
Marendy, D
Gabor, L
George, S D
Parker, A
Doyle, Emma
Publication Date
2023
Abstract
<p>Fasciolosis is an endemic zoonotic parasitic disease with significant impacts on human health and both animal health and production. Early post-infection impacts on the host remain unclear. The objective of this study was to determine the changes, if any, to levels of endotoxin in cattle plasma in response to early-stage infection with <i>Fasciola</i> hepatica. Thirty-six (36) commercial bred cattle were experimentally infected with approximately 400 viable metacercariae. Plasma lipopolysaccharide (endotoxin) levels were examined on 24 occasions from 0 h before infection to 336 h after infection using the Limulus Amoebocyte Lysate chromo-genic end point assay and compared with that of six (6) uninfected control animals. Peak lipopolysaccharide levels in infected animals were reached at 52 h after infection and returned to pre-infection levels at time 144 h after infection. Infected animals had significantly elevated lipopolysaccharide levels between 24 and120 h after infection when compared to uninfected animals. The mean change in endotoxin units (EU)/mL over time after infection was statistically significant in infected animals. Elevations of lipo-polysaccharide occurred in all infected animals suggesting a possible repeatable and titratable endotoxemia conducive to therapeutic agent model development.</p>
Citation
Australian Veterinary Journal, 101(9), p. 334-338
ISSN
1751-0813
0005-0423
Link
Publisher
Wiley-Blackwell Publishing Ltd
Rights
Attribution-NonCommercial-NoDerivatives 4.0 International
Title
Plasma lipopolysaccharide elevations in cattle associated with early-stage infection by Fasciola hepatica
Type of document
Journal Article
Entity Type
Publication

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