In this article, we introduce a novel concept combining neural network technology and Decisional DNA for knowledge representation and sharing. Instead of using traditional machine learning and knowledge discovery methods, this approach explores the way of knowledge extraction through deep learning processes based on a domain’s past decisional events captured by Decisional DNA. We compare our approach with kNN (k-nearest neighbors), logistic regression, and AdaBoost in classification tasks, and the results show that our approach is very promising with regard to the enhancement of the accuracy of knowledge-based predictions required in complex decision-making problems.