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https://hdl.handle.net/1959.11/61721
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DC Field | Value | Language |
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dc.contributor.author | Hossain, Mohammad F | en |
dc.contributor.author | Walkden-Brown, Stephen | en |
dc.date.accessioned | 2024-07-19T02:00:00Z | - |
dc.date.available | 2024-07-19T02:00:00Z | - |
dc.date.issued | 2019 | - |
dc.identifier.uri | https://hdl.handle.net/1959.11/61721 | - |
dc.description | This dataset is related to the publication: <br> Mohammad F. Hossain, Mary McMillan, Margaret E. Katz, Stephen W. Walkden-Brown, Priscilla F. Gerber "Turkey Hemorrhagic Enteritis Virus Can Be Titrated but Not Propagated in Chicken Embryos," Avian Diseases, 63(1), 84-89, (17 December 2018) | en |
dc.description.abstract | This study aimed to investigate the feasibility of propagating and titrating hemorrhagic enteritis virus (HEV) in chicken embryos. A total of 308 embryonated eggs were used. At 10 days of embryonic age, eggs were inoculated via allantoic sac or chorioallantoic membrane routes with non–heat-treated (live) HEV or heat-treated (dead) HEV or served as negative controls. Allantoic fluid retrieved at 0, 1, 3, 5, and 7 days postinoculation (dpi) was tested for HEV by quantitative PCR. Inoculation with HEV did not cause visible growth impairment or lesions in the chicken embryos. Overall, there was no difference in postinoculation mortality rates among groups sham-inoculated (6/30, 20.0%) or inoculated with live (34/252, 13.4%) or dead (3/ 26, 6.9%) HEV (P = 0.58). The amount of HEV DNA detected in allantoic fluid at 7 dpi in eggs inoculated with live virus was similar to the inoculated dose, indicating that virus propagation in chicken embryos is not efficient. No HEV DNA was detected after 3 dpi in eggs inoculated with dead virus. Inoculation of chicken embryos combined with qualitative PCR can be used for titration of HEV virus stocks and presents a high correlation with in vivo titration using chickens (R2 0.98, P = 0.007). This method may be relevant in countries in which specific-pathogen-free turkeys are unavailable and in which the importation of RP19 cells, the only cell that supports effective propagation of HEV, is not permitted. | en |
dc.format.extent | .xlsx | en |
dc.language | en | en |
dc.publisher | University of New England | en |
dc.relation.uri | https://hdl.handle.net/1959.11/26769 | en |
dc.title | Turkey Hemorrhagic Enteritis Virus Can Be Titrated but Not Propagated in Chicken Embryos - Dataset | en |
dc.type | Dataset | en |
dc.identifier.doi | 10.25952/60m1-kt23 | en |
dc.identifier.project | Poultry CRC (project 1.1.9): Vaccine & Diagnostic for Haemorrhagic Enteritis of Turkey | en |
dc.subject.keywords | Turkey adenovirus 3 | en |
dc.subject.keywords | Quantitative PCR | en |
dc.subject.keywords | Virus propagation | en |
dc.subject.keywords | Chickens | en |
local.contributor.firstname | Mohammad F | en |
local.contributor.firstname | Stephen | en |
local.subject.for2008 | 060506 Virology | en |
local.subject.for2008 | 060599 Microbiology not elsewhere classified | en |
local.subject.seo2008 | 860901 Veterinary Biological Preventatives (e.g. Vaccines) | en |
local.dcrelation.contact | Mohammad F Hossain, mhossa29@myune.edu.au | en |
local.dcrelation.custodian | Mohammad F Hossain, mhossa29@myune.edu.au | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.email | swalkden@une.edu.au | en |
local.output.category | X | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.publisher.place | Armidale, Australia | en |
local.contributor.lastname | Hossain | en |
local.contributor.lastname | Walkden-Brown | en |
dc.identifier.staff | une-id:swalkden | en |
local.profile.orcid | 0000-0002-0638-5533 | en |
local.profile.role | creator | en |
local.profile.role | supervisor | en |
local.identifier.unepublicationid | une:1959.11/61721 | en |
dc.identifier.academiclevel | Student | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | Turkey Hemorrhagic Enteritis Virus Can Be Titrated but Not Propagated in Chicken Embryos - Dataset | en |
local.output.categorydescription | X Dataset | en |
local.relation.doi | 10.1637/11982-100918-Reg.1 | en |
local.search.author | Hossain, Mohammad F | en |
local.search.supervisor | Walkden-Brown, Stephen | en |
dcterms.rightsHolder.managedby | Stephen Walkden-Brown | en |
local.datasetcontact.name | Stephen Walkden-Brown | en |
local.datasetcontact.email | swalkden@une.edu.au | en |
local.datasetcustodian.name | Stephen Walkden-Brown | en |
local.datasetcustodian.email | swalkden@une.edu.au | en |
local.datasetcontact.details | Stephen Walkden-Brown - swalkden@une.edu.au | en |
local.datasetcustodian.details | Stephen Walkden-Brown - swalkden@une.edu.au | en |
dcterms.source.datasetlocation | University of New England | en |
local.uneassociation | Unknown | en |
local.atsiresearch | No | en |
local.sensitive.cultural | No | en |
local.year.published | 2019 | en |
local.subject.for2020 | 310706 Virology | en |
local.subject.seo2020 | 241601 Veterinary biological preventatives | en |
local.profile.affiliationtype | UNE Affiliation | en |
local.profile.affiliationtype | UNE Affiliation | en |
Appears in Collections: | Dataset |
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