The online education market share is rapidly increasing, raising the demand to teach sciences outside the laboratory environment. Here, we present Microbiology at Home (M@H), a new approach that integrates hands-on microbiology experimentation with online interactive simulations using authentic scenarios in microbiology in the home environment. The M@H program includes 8 practical activities aligned to the ASM curriculum for practical skills. M@H kits are mailed to students, and each practical activity is prepacked individually with the required consumables, including microbial culture media to prepare at home using a microwave. These practicals are self-paced, and each activity is facilitated using a two-dimensional simulation package with prerecorded videos, protocols, and interactive activities. The students receive both synchronous and asynchronous support and guidance through online learning management systems fora and virtual gatherings. The M@H program was applied to an Introductory Microbiology cohort at the University of New England in 2020 and 2021. Based on student feedback, the experience not only provided real hands-on practice in microbiology but also acted to cement the engagement with the content by contextualizing it to the surrounding home environment. We anticipate that these activities will provide a way to successfully engage students with hands-on microbiology without the need for actual laboratory attendance, thus increasing accessibility to microbial protocols and applications.