Author(s) |
Schaller, Andrea
Connors, Natalie K
Oelmeier, Stefan A
Hubbuch, Jürgen
Middelberg, Anton P J
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Publication Date |
2015-01-06
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Abstract |
<p>Soluble expression of de novo-designed proteins in <i>Escherichia coli</i> (<i>E. coli</i>) remains empirical. For given experimental conditions expression success is determined in part by protein primary sequence. This has been previously explored with varying success using a variety of statistical solubility prediction tools though without taking fold stability into account. In the present study, the three-dimensional structure of proteins in molecular dynamics (MD) simulations is used to predict expression as a new approach with a set of four-helix bundles. Stability-related parameters for ten structures were determined in a thermal unfolding MD simulation and used to build statistical models with a support vector machine (SVM) classifier. The most accurate models were identified by their performance on five independent four-helix bundle sequences. The final model provided accurate classification prediction for this test set and was successfully applied in a model challenge with two newly designed sequences.</p>
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Citation |
Chemical Engineering Science, v.121, p. 340-350
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ISSN |
1873-4405
0009-2509
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Link | |
Publisher |
Elsevier Ltd
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Title |
Predicting recombinant protein expression experiments using molecular dynamics simulation
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Type of document |
Journal Article
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Entity Type |
Publication
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