Tillage and nitrogen fertilization enhanced belowground carbon allocation and plant nitrogen uptake in a semi-arid canola crop–soil system

Abstract

<p>Carbon (C) and nitrogen (N) allocation and assimilation are coupled processes, likely infuencing C accumulation, N use efciency and plant productivity in agro-ecosystems. However, dynamics and responses of these processes to management practices in semi-arid agro-ecosystems are poorly understood. A feld-based ¹³CO₂ and urea-15N pulse labelling experiment was conducted to track how C and N allocation and assimilation during canola growth from fowering to maturity were afected by short-term (2-year) tillage (T) and no-till (NT) with or without 100kg urea-N ha⁻¹ (T-0, T-100, NT-0, NT100) on a Luvisol in an Australian semi-arid region. The T-100 caused greater (P<0.05) belowground C allocation and higher (P<0.05) translocation of soil N to shoots and seeds, compared to other treatments. Microbial N uptake was rapid and greatest in the fertilized (cf. non-fertilized) treatments, followed by a rapid release of microbial immobilized N, thus increasing N availability for plant uptake. In contrast, management practices had insignifcant impact on soil C and N stocks, aggregate stability, microbial biomass, and ¹³C retention in aggregate-size fractions. In conclusion, tillage and N fertilization increased belowground C allocation and crop N uptake and yield, possibly <i>via</i> enhancing root–microbial interactions, with minimal impact on soil properties.</p>