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Title: A preliminary study of the localization of infectious laryngotracheitis virus glycoprotein E within specific peripheral blood lymphocytes
Contributor(s): Nazir, Shahid  (author); Charlesworth, Richard P G  (author)orcid ; Moens, Pierre D J  (author)orcid ; Assen, Awol M (author); Walkden-Brown, Stephen W  (author)orcid ; Freitas Gerber, Priscilla  (author)orcid 
Publication Date: 2022-04
Early Online Version: 2022-01-19
DOI: 10.1080/03079457.2021.2015062
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Infectious laryngotracheitis virus (ILTV) DNA has been detected in blood fractions, but the cell phenotype with which the virus is associated is unknown. This study investigated the presence of ILTV antigen in peripheral blood cells of six acutely ILTV-infected chickens (5 or 9 days post ocular inoculation with a virulent isolate) and three sham-inoculated chickens using immunofluorescent staining. Blood fractions were separated by Ficoll-Paque density gradient centrifugation, and smears were prepared from erythrocyte and leukocyte fractions. The smears were stained for ILTV glycoprotein E and the leukocyte markers CD4, CD8, Bu-1 (B cell), KUL01 (monocyte/macrophage), TCRγδ, and TCRαβ/Vβ2 and examined under a confocal microscope. In samples from infected birds, ILTV gE-specific fluorescence was localized in B cells and all evaluated T cell types, but not in monocytes and erythrocytes. The percentage of CD4, CD8, TCRγδ, TCRαβ/Vβ1, TCRαβ/Vβ2 and B cells positive for ILTV antigen ranged from 13.3% to 22.3%. None of the samples from the sham-inoculated chickens exhibited fluorescence for ILTV gE. The results of this pilot study suggest that ILTV has a tropism for peripheral blood T and B cells. Further research is required to investigate whether these cells support ILTV productive replication.

Publication Type: Journal Article
Grant Details: ARC/DE200101832
Source of Publication: Avian Pathology, 51(2), p. 141-145
Publisher: Taylor & Francis
Place of Publication: United Kingdom
ISSN: 1465-3338
Fields of Research (FoR) 2020: 3009 Veterinary sciences
Socio-Economic Objective (SEO) 2020: TBD
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Journal Article
School of Environmental and Rural Science
School of Science and Technology

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