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Title: Quantitative genotyping to estimate genetic contributions to pooled samples and genetic merit of the contributing entities
Contributor(s): Kinghorn, Brian  (author); Bastiaansen, John W M (author); Ciobanu, Daniel C (author); van der Steen, Hein A M (author)
Publication Date: 2010
DOI: 10.1080/09064701003801922
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Abstract: Genotyping required to track family membership in aquaculture breeding programs is reduced dramatically by estimating the contributions of different families to pooled samples of tissue. This approach is relevant to widely differing scenarios involving animals, plants, and microbes. For the family membership scenario, SNP markers are genotyped for the contributing families’ parents, and quantitatively genotyped to estimate allele frequencies within the mixed-family pooled tissue. Results are used to infer proportional contributions of the different families to the pool. Different computational strategies were tested for bias and sampling error. A correlation of 99% between estimated and true genetic contributions was achieved using 20 (50) randomly chosen SNPs at a standard error of allele frequency estimates of 0.01 (0.02). Optimal grouping of families and choice of markers further increases performance markedly. Trait means and distributions of families can be quite accurately estimated by tissue sampling across the range of trait values.
Publication Type: Journal Article
Source of Publication: Acta Agriculturae Scandinavica, Section A, Animal Science, v.60, p. 3-12
Publisher: Taylor and Francis
Place of Publication: Informa Healthcare
ISSN: 1651-1972
Field of Research (FOR): 060411 Population, Ecological and Evolutionary Genetics
060412 Quantitative Genetics (incl Disease and Trait Mapping Genetics)
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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