Quantitative genotyping to estimate genetic contributions to pooled samples and genetic merit of the contributing entities

Title
Quantitative genotyping to estimate genetic contributions to pooled samples and genetic merit of the contributing entities
Publication Date
2010
Author(s)
Kinghorn, Brian
Bastiaansen, John W M
Ciobanu, Daniel C
van der Steen, Hein A M
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Taylor & Francis Scandinavia
Place of publication
Sweden
DOI
10.1080/09064701003801922
UNE publication id
une:5848
Abstract
Genotyping required to track family membership in aquaculture breeding programs is reduced dramatically by estimating the contributions of different families to pooled samples of tissue. This approach is relevant to widely differing scenarios involving animals, plants, and microbes. For the family membership scenario, SNP markers are genotyped for the contributing families’ parents, and quantitatively genotyped to estimate allele frequencies within the mixed-family pooled tissue. Results are used to infer proportional contributions of the different families to the pool. Different computational strategies were tested for bias and sampling error. A correlation of 99% between estimated and true genetic contributions was achieved using 20 (50) randomly chosen SNPs at a standard error of allele frequency estimates of 0.01 (0.02). Optimal grouping of families and choice of markers further increases performance markedly. Trait means and distributions of families can be quite accurately estimated by tissue sampling across the range of trait values.
Link
Citation
Acta Agriculturae Scandinavica, Section A, Animal Science, v.60, p. 3-12
ISSN
1651-1972
0906-4702
1651-9140
Start page
3
End page
12

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