Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/56808
Title: Host Preference and Thermal Biology of Helicoverpa punctigera (Wallengren) (Lepidoptera: Noctuidae)
Contributor(s): Bawa, Abukari Samuel (author); Andrew, Nigel  (supervisor)orcid ; Del Socorro, Alice  (supervisor); Gregg, Peter  (supervisor)orcid 
Conferred Date: 2021-07-09
Copyright Date: 2021-03-26
Handle Link: https://hdl.handle.net/1959.11/56808
Related DOI: 10.1071/CP20424
10.1111/aen.12563
10.7717/peerj.12479
10.1016/j.jtherbio.2021.103099
Related Research Outputs: https://hdl.handle.net/1959.11/56809
Abstract: 

The native budworm Helicoverpa punctigera (Wallengren) and cotton bollworm Helicoverpa armigera (Hübner) are important insect pests of field and horticultural crops in Australia. H. armigera is a well-studied, cosmopolitan species, whereas H. punctigera is a widely distributed Australian native with little knowledge of its general ecology. It is believed that native noncrop host plants in the inland regions of the continent could be possible sources of H. punctigera moths migrating to the south-eastern cropping regions as larvae have been recorded on these native non-crop hosts in the inland regions. Plants with the C4 photosynthetic pathway are among the dominant native non-crop hosts in inland regions of Australia. However, there has been little work on their occurrence and successful development on these non-crop hosts, especially on plants with the C4 photosynthetic pathway.

My thesis reports aspects of H. punctigera ecology to improve our understanding of its population dynamics especially in the inland regions. I assessed adult oviposition preference, larval survival and adult oviposition response of H. punctigera on some plants with the C4 photosynthetic pathway, compared with C3 plants under glasshouse conditions. In addition, because inland Australia is characterised by high and extreme temperatures in summer, I assessed the physiological responses of the insect to exposure to both high and extreme temperatures.

For oviposition studies, I conducted two-way and multi-choice tests on two C4 plants (Atriplex nummularia Lindl. and Atriplex vesicaria Heward ex Benth), against two C3 plants (Medicago polymorpha L. and Cullen cinereum (Lindl.) J.W.Grimes). Although oviposition occurred on both the C3 and C4 plants, ovipositing females showed preference for the C3 plants vs the C4 plants. In addition, females preferred Atriplex nummularia against Atriplex vesicaria in both a multi-and two-choice test, and preferred the adaxial and abaxial of Atriplex nummularia and Atriplex vesicaria respectively as their oviposition site.

There are few records of H. punctigera survival on non-crop hosts especially on plants with the C4 photosynthetic pathway. Larval performance was investigated on four C4 plant species (Atriplex numularia Lindl. Atriplex vesicaria Heward ex Benth., Portulaca oleraceae L. and Tribulus terrestris L.) and two C3 plant species (Medicago polymorpha L. and Cullen cinereum (Lindl.)). Larvae survived on all the C4 plants, with high survival (32.2±6.9 and 31.7±6.9 percent survival) on both Atriplex species and lowest survival (15.9±7.9 percent survival) was on P. oleraceae. Larval survival on the C4 plant A. nummularia, was significantly higher than on both the C4 plant A. vesicaria and the C3 plant M. polymorpha.

Ovipositing plant-feeding insect species use volatile organic compounds released by their host plants to recognise their hosts and select oviposition sites for egg deposition. By assessing the oviposition response of ovipositing H. punctigera females to leaf extracts of two C4 plants, I found in both two-way and multi-way oviposition response bioassays, substrates treated with extracts of the C4 plants attracted more females than untreated controls, suggesting that the plants may contain a compounds that can influence gravid H. punctigera. Leaf extracts of Atriplex nummularia received significantly more eggs compared with extracts of Atriplex vesicaria.

A warmer and drier climate is predicted to become more extreme into the future exposing terrestrial insects to further thermal stress. On this premise, I assessed the thermal physiology (critical thermal maximum) of H. punctigera across three life stages (larvae, pupae and adults) using thermolimit respirometry assays to gain an understanding of the impact of rising temperature on the insect. Critical thermal maximum significantly differed among the life stages with larvae having the highest thermal maximum (49.1°C±0.3°C) compared with pupae (47.3°C ±0.2°C) and adults (46.9°C±0.2°C).

High temperatures in the field, which occur as single, prolonged, or repeated small-scale events, can affect the population performance of insects. Assessing the impact of different heat stress regimes can provide important information on the biological performance of H. punctigera under climate change. Here, I subjected H. punctigera pupae to two extreme temperatures – 44.2°C and 43°C which are just below the CTmax and two exposure treatments (single prolonged exposure and repeated short exposure), to assess the biological traits of individuals. Heat stress on pupae resulted in reduced fecundity by 50%, nil egg hatchability, extended pre-oviposition period by one day, and adult deformities.

This thesis has broadened our understanding that Helicoverpa punctigera could utilize plants with the C4 photosynthetic pathways that are common in inland regions of Australia. This is a significant contribution to our understanding of the ecology and biology of H. punctigera.

Publication Type: Thesis Doctoral
Fields of Research (FoR) 2008: 060808 Invertebrate Biology
Socio-Economic Objective (SEO) 2008: 960403 Control of Animal Pests, Diseases and Exotic Species in Farmland, Arable Cropland and Permanent Cropland Environments
Socio-Economic Objective (SEO) 2020: 180602 Control of pests, diseases and exotic species in terrestrial environments
HERDC Category Description: T2 Thesis - Doctorate by Research
Description: Please contact rune@une.edu.au if you require access to this thesis for the purpose of research or study.
Appears in Collections:School of Environmental and Rural Science
School of Science and Technology
Thesis Doctoral

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