Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/5580
Title: In vitro fermentation if fructo-oligosaccharides and trans galacto-oligosaccharides by adapted and unadapted bacterial populations for the gastrointestinal tract of piglets
Contributor(s): Mikkelsen, Lene Lind  (author); Knudsen, Knud Eric Bach (author); Jensen, Bent Borg (author)
Publication Date: 2004
DOI: 10.1016/j.anifeedsci.2004.07.007
Handle Link: https://hdl.handle.net/1959.11/5580
Abstract: An in vitro study was performed to investigate the fermentation of fructo-oligosaccharides (FOS) and transgalacto-oligosaccharides (TOS) by bacteria harvested from the gastrointestinal tract of piglets. In addition, the apparent ileal digestibility of FOS and TOS was measured. Thirty piglets were fed either a semi-synthetic control diet or the control diet supplemented with 40 g FOS or 40 g TOS per kg (n = 10). After 29 days, the piglets were killed and the gastrointestinal contents collected for the in vitro experiment and chemical analysis. The results demonstrated that bacteria from the stomach were nearly incapable of degrading FOS and TOS, whereas bacteria from the distal small intestine to some extend fermented FOS and TOS. Fermentation of FOS and TOS with bacteria from the stomach and distal small intestine was not affected by the dietary treatment of the piglets. Bacteria from the caecum and mid colon of the piglets showed the highest capacity to degrade FOS and TOS in vitro and the microbiota seemed to adapt to the dietary FOS. Caecal bacteria from piglets fed the FOS diet had tendencies to increased production rate of organic acids (P < 0.1), propionic acid (P < 0.1) and valeric acid (P < 0.1) and significantly higher production rate of butyric acid (P < 0.05) from FOS as compared with bacteria from piglets fed the control and TOS diet. Colonic bacteria from piglets fed the FOS diet also had significantly higher production rates of butyric acid (P < 0.05) and capronic acid (P < 0.01). In addition, significantly lower molar proportions of acetic acid (P < 0.05) and propionic acid (P < 0.05), and a significantly higher molar proportion of capronic acid (P < 0.05) were observed for colonic bacteria from piglets fed the FOS diet as compared with the control diet. Fermentation of TOS by bacteria from the caecum and mid colon was generally not affected by the dietary treatment of the piglets. It was estimated that the capacity for microbial degradation of the dietary FOS and TOS during passage of the stomach and small intestine of the piglets was approximately a proportion of 0.20 for FOS and 0.16 for TOS. The coefficient of apparent ileal digestibility (CAID) was 0.57 (range 0.39–0.89) for FOS and 0.56 (range 0.31–0.82) for TOS. In conclusion, a proportion between 0.20 and 0.60 of FOS and TOS are degraded by the microbiota in the small intestine of piglets and the microbiota of the large intestine adapt to the administration of FOS in the feed.
Publication Type: Journal Article
Source of Publication: Animal Feed Science and Technology, 116(3-4), p. 225-238
Publisher: Elsevier BV
Place of Publication: Amsterdam, The Netherlands
ISSN: 1873-2216
0377-8401
Field of Research (FOR): 060504 Microbial Ecology
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Statistics to Oct 2018: Visitors: 82
Views: 82
Downloads: 0
Appears in Collections:Journal Article

Files in This Item:
2 files
File Description SizeFormat 
Show full item record

SCOPUSTM   
Citations

15
checked on Nov 26, 2018

Page view(s)

36
checked on Mar 1, 2019
Google Media

Google ScholarTM

Check

Altmetric


Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.