Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/5523
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dc.contributor.authorMacMillan, Keithen
dc.contributor.authorBlok, Vivianen
dc.contributor.authorYoung, Iainen
dc.contributor.authorCrawford, Johnen
dc.contributor.authorWilson, Michael Jen
dc.date.accessioned2010-04-13T09:16:00Z-
dc.date.issued2006-
dc.identifier.citationInternational Journal for Parasitology, 36(14), p. 1453-1461en
dc.identifier.issn1879-0135en
dc.identifier.issn0020-7519en
dc.identifier.urihttps://hdl.handle.net/1959.11/5523-
dc.description.abstract'Phasmarhabditis hermaphrodita' is a nematode parasite that infects and kills several species of slugs. The nematode is produced commercially as a biological control agent for slug pests of agriculture and horticulture. Given the difficulties of distinguishing this species from other nematode species in soil samples, very little is known about its natural ecology or its behaviour and persistence following application for biological control. Here we describe a method to quantify 'P. hermaphrodita' in soil samples based on real time PCR. We designed primers and a dual labelled fluorescent probe that can be used to quantify numbers of 'P. hermaphrodita' and which is capable of distinguishing this species from the morphologically identical 'Phasmarhabditis neopapillosa'. We compared different methods whereby the entire nematode community is extracted prior to DNA extraction, and three methods to extract DNA directly from soil samples. Both nematode extraction and DNA extraction from large (10 g) samples of soil gave reliable estimates of nematode numbers, but methods which extracted DNA from small (1 g or less) soil samples substantially underestimated numbers. However, direct extraction of DNA from soils may overestimate numbers of live nematodes as DNA from dead nematodes was found to persist in soil for at least 6 days. The technique could be modified for detection and quantification of all soil borne parasitic nematodes.en
dc.languageenen
dc.publisherElsevier Ltden
dc.relation.ispartofInternational Journal for Parasitologyen
dc.titleQuantification of the slug parasitic nematode 'Phasmarhabditis hermaphrodita' from soil samples using real time qPCRen
dc.typeJournal Articleen
dc.identifier.doi10.1016/j.ijpara.2006.08.005en
dc.subject.keywordsSoil Physicsen
dc.subject.keywordsTerrestrial Ecologyen
dc.subject.keywordsSoil Biologyen
dc.subject.keywordsSoil Sciencesen
local.contributor.firstnameKeithen
local.contributor.firstnameVivianen
local.contributor.firstnameIainen
local.contributor.firstnameJohnen
local.contributor.firstnameMichael Jen
local.subject.for2008050303 Soil Biologyen
local.subject.for2008050305 Soil Physicsen
local.subject.for2008050399 Soil Sciences not elsewhere classifieden
local.subject.for2008060208 Terrestrial Ecologyen
local.subject.seo2008961402 Farmland, Arable Cropland and Permanent Cropland Soilsen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.emailiyoung4@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordune-20100225-112612en
local.publisher.placeUnited Kingdomen
local.format.startpage1453en
local.format.endpage1461en
local.peerreviewedYesen
local.identifier.volume36en
local.identifier.issue14en
local.contributor.lastnameMacMillanen
local.contributor.lastnameBloken
local.contributor.lastnameYoungen
local.contributor.lastnameCrawforden
local.contributor.lastnameWilsonen
dc.identifier.staffune-id:iyoung4en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:5654en
dc.identifier.academiclevelAcademicen
local.title.maintitleQuantification of the slug parasitic nematode 'Phasmarhabditis hermaphrodita' from soil samples using real time qPCRen
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.search.authorMacMillan, Keithen
local.search.authorBlok, Vivianen
local.search.authorYoung, Iainen
local.search.authorCrawford, Johnen
local.search.authorWilson, Michael Jen
local.uneassociationUnknownen
local.year.published2006en
Appears in Collections:Journal Article
School of Environmental and Rural Science
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