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Title: Root cap influences root colonisation by 'Pseudomonas fluorescens' SBW25 on maize
Contributor(s): Humphris, S N (author); Bengough, A G (author); Griffiths, B S (author); Kilham, B S (author); Rodger, S (author); Stubbs, V (author); Valentine, T (author); Young, I  (author)
Publication Date: 2005
DOI: 10.1016/j.femsec.2005.03.005
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Abstract: We investigated the influence of root border cells on the colonisation of seedling 'Zea mays' roots by 'Pseudomonas flourescens' SBW25 in sandy loam soil packed at two dry bulk densities. Numbers of colony forming units (CFU) were counted on sequential sections of root for intact and decapped inoculated roots grown in loose (1.0mg m-3) and compacted (1.3mg m⁻³) soil. After two days of root growth, the numbers of 'P. flourescens' (CFU cm⁻¹) were highest on the section of root just below the seed with progressively fewer bacteria near the tip, irrespective of density. The decapped roots had significantly more colonies of 'P. flourescens' at the tip compared with the intact roots: approximately 100-fold more in the loose and 30-fold more in the compact soil. In addition, confocal images of the root tips grown in agar showed that 'P. flourescens' could only be detected on the tips of the decapped roots. These results indicated that border cells, and their associated mucilage, prevented complete colonization of the root tip by the biocontrol agent 'P. flourescens', possibly by acting as a disposable surface or sheath around the cap.
Publication Type: Journal Article
Source of Publication: FEMS Microbiology Ecology, v.54, p. 123-130
Publisher: Wiley-Blackwell Publishing Ltd
Place of Publication: United Kingdom
ISSN: 0168-6496
Field of Research (FOR): 050303 Soil Biology
060208 Terrestrial Ecology
050399 Soil Sciences not elsewhere classified
050305 Soil Physics
Socio-Economic Outcome Codes: 961402 Farmland, Arable Cropland and Permanent Cropland Soils
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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