The aim of this study was to identify quantitative trait loci (QTL) associated with residual feed intake (RFI) and genes whose expression varied significantly with phenotypic differences in RFI. We used data from 2,190 Angus steers with phenotypic records for RFI, all with imputed high density array (770k) genotypes. We used RNA-seq in a multi-tissue experiment from 126 Angus steers divergently selected for RFI for approximately three generations, to analyze the expression of genes significantly associated (GSA) with RFI, with special attention to the genes close by significant QTLs.