A molecular based method for rapid detection of Salmonella spp. in poultry dust samples

Abstract

<p>Salmonellosis, caused by <i>Salmonella</i> spp., is a widely reported foodborne zoonosis frequently associated with ingestion of poultry products. <i>Salmonella</i> vaccination of chickens can be used to reduce bacterial shedding and risk of human infection. To determine <i>Salmonella</i> burden in chicken farms, culture methods of environmental samples that require a turn-around time of 5-7 days are usually used. Rapid screening using molecular assays such as PCR of pre-enriched broth has been reported for <i>Salmonella</i> spp. detection in feed, floor dust, and drag swabs within 2-3 days. Here we report an adaptation of the method for detection of <i>Salmonella</i> in poultry dust samples collected using a settle plate method under experimental conditions. Key features:</p> <ul><li>Passive dust sample collection using dry settle plates without media suspended from dropper lines of drinkers.</li><li>Small amount of sample required for the pre-enrichment process.</li><li>Quantification of Salmonella DNA with high sensitivity using an inexpensive extraction protocol.</li></ul>