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A collection of 113,497 public Expressed Sequence Tags (ESTs) from Genbank dbEST were utilized. Nucleotide similarities between pig ESTs and human genes were determined by cDNA alignment against 97,100 human UniGene sequences. Available data for pig-on-human comparative maps and homology between pig and human chromosomes were then used to predict pig EST assignments on pig chromosomes. The similarities among pig ESTs and 3,331 pig genes retrieved from GenBank were also examined. Results showed that 28,113 pig ESTs hits 10,258 human UniGenes, and 6,254 pig ESTs hits 1,362 pig gene sequences (score >= 200). As a results of this comparative analysis, a porcine expressed sequence tagged (EST) database, a set of tools for EST analyses and a web query tool for public access to this database were developed. This database is comprised of a number of tables covering the EST profile, alignment similarity, human ortholog information on cytogenetic, and RH locations. Interactive web query interfaces are developed for public access to the EST database. |
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