Differentiation of Marek's diesease virus serotypes using PCR: Research and field experience

Title
Differentiation of Marek's diesease virus serotypes using PCR: Research and field experience
Publication Date
2003
Author(s)
Walkden-Brown, Steve William
( author )
OrcID: https://orcid.org/0000-0002-0638-5533
Email: swalkden@une.edu.au
UNE Id une-id:swalkden
Groves, P J
Islam, Afm Fakhrul
Burgess, S K
Arzey, K E
Mascord, L
Young, P L
Editor
Editor(s): RAE Pym
Type of document
Conference Publication
Language
en
Entity Type
Publication
Publisher
University of Sydney, Poultry Research Foundation
Place of publication
Sydney, Australia
UNE publication id
une:4429
Abstract
PCR assays for serotypes 1, 2 and 3 Marek's disease virus (MDV) and their performance under experimental and field conditions, are described. The assays appear to be serotype-specific and are of broadly similar sensitivity as virus isolation and identification with immunostaining. They offer significant advantages over virus isolation in speed and cost of diagnosis, handling and storage of tissues for assay, and ability to discriminate between serotypes in mixed infections. Limitations include the ongoing requirement for invasive tissue sampling, the lack of quantification of viral load, inability to detect early MDV-1 infections in HVT-vaccinated birds and the inability to differentiate between oncogenic and attenuated vaccinal strains of MDV-1.
Link
Citation
Proceedings of the Australian Poultry Science Symposium, v.15, p. 192-196
ISSN
1034-6260
1034-3466
Start page
192
End page
196

Files:

NameSizeformatDescriptionLink