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|Title:||Differentiation of Marek's diesease virus serotypes using PCR: Research and field experience||Contributor(s):||Walkden-Brown, Steve William (author) ; Groves, P J (author); Islam, Afm Fakhrul (author); Burgess, S K (author); Arzey, K E (author); Mascord, L (author); Young, P L (author)||Publication Date:||2003||Handle Link:||https://hdl.handle.net/1959.11/4327||Abstract:||PCR assays for serotypes 1, 2 and 3 Marek's disease virus (MDV) and their performance under experimental and field conditions, are described. The assays appear to be serotype-specific and are of broadly similar sensitivity as virus isolation and identification with immunostaining. They offer significant advantages over virus isolation in speed and cost of diagnosis, handling and storage of tissues for assay, and ability to discriminate between serotypes in mixed infections. Limitations include the ongoing requirement for invasive tissue sampling, the lack of quantification of viral load, inability to detect early MDV-1 infections in HVT-vaccinated birds and the inability to differentiate between oncogenic and attenuated vaccinal strains of MDV-1.||Publication Type:||Conference Publication||Conference Details:||Australian Poultry Science Symposium, Sydney, Australia, 10 - 12 February, 2003||Source of Publication:||Proceedings of Australian Poultry Science Symposium, v.15, p. 192-196||Publisher:||Australian Poultry Science Symposium & Poultry Research Foundation & World's Poultry Science Association, Australian Branch||Place of Publication:||Sydney, NSW, Australia||ISSN:||1034-6260
|Field of Research (FOR):||070712 Veterinary Virology||Socio-Economic Objective (SEO):||830309 Poultry||Peer Reviewed:||Yes||HERDC Category Description:||E1 Refereed Scholarly Conference Publication||Other Links:||http://trove.nla.gov.au/work/6382329?selectedversion=NBD7527026
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|Appears in Collections:||Conference Publication|
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