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Title: Differentiation of Marek's diesease virus serotypes using PCR: Research and field experience
Contributor(s): Walkden-Brown, Steve William  (author)orcid ; Groves, P J (author); Islam, Afm Fakhrul  (author); Burgess, S K (author); Arzey, K E (author); Mascord, L (author); Young, P L (author)
Publication Date: 2003
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Abstract: PCR assays for serotypes 1, 2 and 3 Marek's disease virus (MDV) and their performance under experimental and field conditions, are described. The assays appear to be serotype-specific and are of broadly similar sensitivity as virus isolation and identification with immunostaining. They offer significant advantages over virus isolation in speed and cost of diagnosis, handling and storage of tissues for assay, and ability to discriminate between serotypes in mixed infections. Limitations include the ongoing requirement for invasive tissue sampling, the lack of quantification of viral load, inability to detect early MDV-1 infections in HVT-vaccinated birds and the inability to differentiate between oncogenic and attenuated vaccinal strains of MDV-1.
Publication Type: Conference Publication
Conference Details: Australian Poultry Science Symposium, Sydney, Australia, 10 - 12 February, 2003
Source of Publication: Proceedings of Australian Poultry Science Symposium, v.15, p. 192-196
Publisher: Australian Poultry Science Symposium & Poultry Research Foundation & World's Poultry Science Association, Australian Branch
Place of Publication: Sydney, NSW, Australia
ISSN: 1034-6260
Field of Research (FOR): 070712 Veterinary Virology
Socio-Economic Objective (SEO): 830309 Poultry
Peer Reviewed: Yes
HERDC Category Description: E1 Refereed Scholarly Conference Publication
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