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https://hdl.handle.net/1959.11/4326
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DC Field | Value | Language |
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dc.contributor.author | Choct, Mingan | en |
dc.contributor.author | Nhan, U | en |
dc.contributor.author | Kocher, Andreas | en |
dc.contributor.author | Tan, H M | en |
dc.contributor.author | Teo, A | en |
dc.contributor.author | Carter, R R | en |
local.source.editor | Editor(s): TA Scott | en |
dc.date.accessioned | 2010-01-28T14:47:00Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | Proceedings of the Australian Poultry Science Symposium, v.16, p. 43-46 | en |
dc.identifier.issn | 1034-3466 | en |
dc.identifier.issn | 1034-6260 | en |
dc.identifier.uri | https://hdl.handle.net/1959.11/4326 | - |
dc.description.abstract | Two enzyme assays, the radial diffusion and the dye-release methods, were modified and tested for their suitability in measuring β-glucanase activity in poultry feed. Enzymes A and B were incorporated into a barley-based broiler starter diet at three levels (500, 1000 and 2000 g/t). The measured β-glucanase activities were 35 U/g and 49 u/g for Enzymes A and B, respectively. A comparison of enzyme recovery rates from samples containing known amounts of the enzyme and from a buffer solution containing the same amount of the enzyme revealed that a fixed percentage of the enzyme bound to feed components in a dose responsive manner. Thus, enzyme recovery rates for the feed samples were not complete and varied between 66% and 97% for the radial diffusion assay and between 76% and 91% for the dyerelease assay. The two assays largely agreed with each other although at low enzyme concentrations, the dye-release assays gave higher recovery rates with lower standard errors. It may be concluded that both assays are useful in determining β-glucanase activity in feed and digesta samples, but the radial diffusion assay is cheaper to perform. | en |
dc.language | en | en |
dc.publisher | University of Sydney, Poultry Research Foundation | en |
dc.relation.ispartof | Proceedings of the Australian Poultry Science Symposium | en |
dc.title | The accuracy and usability of the radial gel diffusion assay and a dye-release technique for determination of β-glucanase in feed | en |
dc.type | Conference Publication | en |
dc.relation.conference | APSS 2004: 16th Annual Australian Poultry Science Symposium | en |
dc.subject.keywords | Animal Nutrition | en |
local.contributor.firstname | Mingan | en |
local.contributor.firstname | U | en |
local.contributor.firstname | Andreas | en |
local.contributor.firstname | H M | en |
local.contributor.firstname | A | en |
local.contributor.firstname | R R | en |
local.subject.for2008 | 070204 Animal Nutrition | en |
local.subject.seo2008 | 830309 Poultry | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.school | School of Environmental and Rural Science | en |
local.profile.email | mchoct@une.edu.au | en |
local.profile.email | akocher2@une.edu.au | en |
local.output.category | E1 | en |
local.record.place | au | en |
local.record.institution | University of New England | en |
local.identifier.epublicationsrecord | pes:1763 | en |
local.date.conference | 9th - 11th February, 2004 | en |
local.conference.place | Sydney, Australia | en |
local.publisher.place | Sydney, Australia | en |
local.format.startpage | 43 | en |
local.format.endpage | 46 | en |
local.peerreviewed | Yes | en |
local.identifier.volume | 16 | en |
local.contributor.lastname | Choct | en |
local.contributor.lastname | Nhan | en |
local.contributor.lastname | Kocher | en |
local.contributor.lastname | Tan | en |
local.contributor.lastname | Teo | en |
local.contributor.lastname | Carter | en |
dc.identifier.staff | une-id:mchoct | en |
dc.identifier.staff | une-id:akocher2 | en |
local.profile.orcid | 0000-0002-2242-8222 | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.profile.role | author | en |
local.identifier.unepublicationid | une:4428 | en |
dc.identifier.academiclevel | Academic | en |
dc.identifier.academiclevel | Academic | en |
local.title.maintitle | The accuracy and usability of the radial gel diffusion assay and a dye-release technique for determination of β-glucanase in feed | en |
local.output.categorydescription | E1 Refereed Scholarly Conference Publication | en |
local.relation.url | http://trove.nla.gov.au/work/6382329?selectedversion=NBD7527026 | en |
local.relation.url | http://www.vetsci.usyd.edu.au/apss/documents/2004/APSS2004-choct-pp43-46.pdf | en |
local.conference.details | APSS 2004: 16th Annual Australian Poultry Science Symposium, Sydney, Australia, 9 - 11 February, 2004 | en |
local.search.author | Choct, Mingan | en |
local.search.author | Nhan, U | en |
local.search.author | Kocher, Andreas | en |
local.search.author | Tan, H M | en |
local.search.author | Teo, A | en |
local.search.author | Carter, R R | en |
local.uneassociation | Unknown | en |
local.year.published | 2004 | en |
local.date.start | 2004-02-09 | - |
local.date.end | 2004-02-11 | - |
Appears in Collections: | Conference Publication |
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