Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/4326
Title: The accuracy and usability of the radial gel diffusion assay and a dye-release technique for determination of β-glucanase in feed
Contributor(s): Choct, Mingan (author)orcid ; Nhan, U (author); Kocher, Andreas (author); Tan, H M (author); Teo, A (author); Carter, R R (author)
Publication Date: 2004
Handle Link: https://hdl.handle.net/1959.11/4326
Abstract: Two enzyme assays, the radial diffusion and the dye-release methods, were modified and tested for their suitability in measuring β-glucanase activity in poultry feed. Enzymes A and B were incorporated into a barley-based broiler starter diet at three levels (500, 1000 and 2000 g/t). The measured β-glucanase activities were 35 U/g and 49 u/g for Enzymes A and B, respectively. A comparison of enzyme recovery rates from samples containing known amounts of the enzyme and from a buffer solution containing the same amount of the enzyme revealed that a fixed percentage of the enzyme bound to feed components in a dose responsive manner. Thus, enzyme recovery rates for the feed samples were not complete and varied between 66% and 97% for the radial diffusion assay and between 76% and 91% for the dyerelease assay. The two assays largely agreed with each other although at low enzyme concentrations, the dye-release assays gave higher recovery rates with lower standard errors. It may be concluded that both assays are useful in determining β-glucanase activity in feed and digesta samples, but the radial diffusion assay is cheaper to perform.
Publication Type: Conference Publication
Conference Name: Australian Poultry Science Symposium, Sydney, Australia, 9 - 11 February, 2004
Source of Publication: Proceedings of Australian Poultry Science Symposium, v.16, p. 43-46
Publisher: Australian Poultry Science Symposium & Poultry Research Foundation & World's Poultry Science Association, Australian Branch
Place of Publication: Sydney, NSW, Australia
ISSN: 1034-3466
1034-6260
Field of Research (FOR): 070204 Animal Nutrition
Peer Reviewed: Yes
HERDC Category Description: E1 Refereed Scholarly Conference Publication
Other Links: http://trove.nla.gov.au/work/6382329?selectedversion=NBD7527026
http://www.vetsci.usyd.edu.au/apss/documents/2004/APSS2004-choct-pp43-46.pdf
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