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Title: The accuracy and usability of the radial gel diffusion assay and a dye-release technique for determination of β-glucanase in feed
Contributor(s): Choct, Mingan  (author)orcid ; Nhan, U (author); Kocher, Andreas (author); Tan, H M (author); Teo, A (author); Carter, R R (author)
Publication Date: 2004
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Abstract: Two enzyme assays, the radial diffusion and the dye-release methods, were modified and tested for their suitability in measuring β-glucanase activity in poultry feed. Enzymes A and B were incorporated into a barley-based broiler starter diet at three levels (500, 1000 and 2000 g/t). The measured β-glucanase activities were 35 U/g and 49 u/g for Enzymes A and B, respectively. A comparison of enzyme recovery rates from samples containing known amounts of the enzyme and from a buffer solution containing the same amount of the enzyme revealed that a fixed percentage of the enzyme bound to feed components in a dose responsive manner. Thus, enzyme recovery rates for the feed samples were not complete and varied between 66% and 97% for the radial diffusion assay and between 76% and 91% for the dyerelease assay. The two assays largely agreed with each other although at low enzyme concentrations, the dye-release assays gave higher recovery rates with lower standard errors. It may be concluded that both assays are useful in determining β-glucanase activity in feed and digesta samples, but the radial diffusion assay is cheaper to perform.
Publication Type: Conference Publication
Conference Details: APSS 2004: 16th Annual Australian Poultry Science Symposium, Sydney, Australia, 9 - 11 February, 2004
Source of Publication: Proceedings of the Australian Poultry Science Symposium, v.16, p. 43-46
Publisher: University of Sydney, Poultry Research Foundation
Place of Publication: Sydney, Australia
ISSN: 1034-3466
Fields of Research (FoR) 2008: 070204 Animal Nutrition
Socio-Economic Objective (SEO) 2008: 830309 Poultry
Peer Reviewed: Yes
HERDC Category Description: E1 Refereed Scholarly Conference Publication
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Appears in Collections:Conference Publication

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