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|Title:||The accuracy and usability of the radial gel diffusion assay and a dye-release technique for determination of β-glucanase in feed||Contributor(s):||Choct, Mingan (author) ; Nhan, U (author); Kocher, Andreas (author); Tan, H M (author); Teo, A (author); Carter, R R (author)||Publication Date:||2004||Handle Link:||https://hdl.handle.net/1959.11/4326||Abstract:||Two enzyme assays, the radial diffusion and the dye-release methods, were modified and tested for their suitability in measuring β-glucanase activity in poultry feed. Enzymes A and B were incorporated into a barley-based broiler starter diet at three levels (500, 1000 and 2000 g/t). The measured β-glucanase activities were 35 U/g and 49 u/g for Enzymes A and B, respectively. A comparison of enzyme recovery rates from samples containing known amounts of the enzyme and from a buffer solution containing the same amount of the enzyme revealed that a fixed percentage of the enzyme bound to feed components in a dose responsive manner. Thus, enzyme recovery rates for the feed samples were not complete and varied between 66% and 97% for the radial diffusion assay and between 76% and 91% for the dyerelease assay. The two assays largely agreed with each other although at low enzyme concentrations, the dye-release assays gave higher recovery rates with lower standard errors. It may be concluded that both assays are useful in determining β-glucanase activity in feed and digesta samples, but the radial diffusion assay is cheaper to perform.||Publication Type:||Conference Publication||Conference Name:||Australian Poultry Science Symposium, Sydney, Australia, 9 - 11 February, 2004||Conference Details:||Australian Poultry Science Symposium, Sydney, Australia, 9 - 11 February, 2004||Source of Publication:||Proceedings of Australian Poultry Science Symposium, v.16, p. 43-46||Publisher:||Australian Poultry Science Symposium & Poultry Research Foundation & World's Poultry Science Association, Australian Branch||Place of Publication:||Sydney, NSW, Australia||ISSN:||1034-3466
|Field of Research (FOR):||070204 Animal Nutrition||Peer Reviewed:||Yes||HERDC Category Description:||E1 Refereed Scholarly Conference Publication||Other Links:||http://trove.nla.gov.au/work/6382329?selectedversion=NBD7527026
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