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Title: Monitoring Marek's disease virus in broiler flocks using standard and real-time quantitative PCR of spleen and dust samples
Contributor(s): Walkden-Brown, Steve William  (author)orcid ; Groves, P J (author); Islam, Aminul (author); Rubite, A T (author); Islam, A Fakhrul  (author); Burgess, Susan  (author)
Publication Date: 2004
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Abstract: We report the monitoring of Marek's Disease virus (MDV) on two unvaccinated commercial broiler farms using standard and real-time polymerase chain reaction (PCR). Standard (non-quantitative) PCR for MDV in spleen samples from five birds per shed (three sheds per farm), sampled at approximately weekly intervals, was compared with standard PCR of DNA extracted from shed dust, and quantitative real-time PCR (qPCR) of the same dust samples. The two farms could not be easily differentiated on the basis of standard PCR tests of spleen with the first positive spleens detected at days 27 and 25 on Farms A and B respectively, and 100% positives in a shed reached on days 41 and 32 respectively. Dust became positive to standard PCR at days 34 and 19 (one shed only) respectively for the two farms suggesting a greater MDV load on Farm B. Real-time qPCR of dust samples detected MDV in all dust samples (earliest day 19) and showed an exponential increase in virus content over time. It also revealed clear differences between farms with dust from Farm B containing approximately 10 times more MDV than that of Farm A. This was associated with reduced performance on Farm B as determined by FCR (2.086 v 2.203 for A and B respectively) and late mortality after day 28 (1.96 v 4.03%). These preliminary data suggest that q-PCR of poultry dust will prove useful for the routine monitoring of MD status in broiler flocks.
Publication Type: Conference Publication
Conference Details: 16th Annual Australian Poultry Science Symposium, Sydney, Australia, 9th - 11th February, 2004
Source of Publication: Proceedings of the Australian Poultry Science Symposium, v.16, p. 149-152
Publisher: Australian Poultry Science Symposium & Poultry Research Foundation & World's Poultry Science Association, Australian Branch
Place of Publication: Sydney, Australia
ISSN: 1034-3466
Field of Research (FOR): 070205 Animal Protection (Pests and Pathogens)
Socio-Economic Objective (SEO): 830309 Poultry
Peer Reviewed: Yes
HERDC Category Description: E1 Refereed Scholarly Conference Publication
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School of Environmental and Rural Science

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