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Title: Investigation of methaemoglobin reduction by extracellular NADH in mammalian erythrocytes
Contributor(s): Kennett, Eleanor C (author); Ogawa, Eri (author); Agar, Nihal S (author); Godwin, Ian Robert  (author); Bubb, William A (author); Kuchel, Philip W (author)
Publication Date: 2005
DOI: 10.1016/j.biocel.2005.02.001
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Abstract: The effect of extracellular NADH on the rate of reduction of nitrite-induced methaemoglobin in erythrocytes from man, cattle, dog, horse, grey kangaroo, pig and sheep was investigated. Extracellular NADH was found to enhance the rate of methaemoglobin reduction in man, dog, pig and kangaroo erythrocytes, but had essentially no effect on the rate of methaemoglobin reduction in erythrocytes from cattle, horse and sheep. In erythrocytes of those animals affected by extracellular NADH the rate of reduction of metHb in the presence of NADH was the same or greater than that observed in the presence of nutrients such as glucose and inosine. The combination of nutrient and NADH produced a more profound increase in the rate of methaemoglobin reduction. The rate of methaemoglobin reduction in all cases was significantly less than that observed with methylene blue, the standard treatment of methaemoglobinaemia. Extracellular NADH was found to indirectly increase the intracellular NADH concentration through displacement of the pseudo-equilibrium of the intracellular LDH reaction and relied upon the presence of sufficient LDH activity released into the extracellular medium through haemolysis. The lack of response of cattle, horse and sheep RBCs to extracellular NADH was found to derive mainly from their low extracellular LDH activity, but also correlated with their lower NADH-methaemoglobin reductase activity compared to the other species. © 2005 Elsevier Ltd. All rights reserved.
Publication Type: Journal Article
Source of Publication: The International Journal of Biochemistry and Cell Biology, 37(7), p. 1438-1445
Publisher: Elsevier
Place of Publication: United Kingdom
ISSN: 1878-5875
Field of Research (FOR): 070204 Animal Nutrition
Socio-Economic Outcome Codes: 830311 Sheep - Wool
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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