Isolation of the Bacteriophage DinoHI from 'Dichelobacter nodosus' and its Interactions with other Integrated Genetic Elements

Title
Isolation of the Bacteriophage DinoHI from 'Dichelobacter nodosus' and its Interactions with other Integrated Genetic Elements
Publication Date
2008
Author(s)
Cheetham, Brian Frederick
Parker, Dane
Bloomfield, Garry A
Shaw, Bruce E
Sutherland, Megan Morag
Hyman, Jessica
Druitt, Jenifer Hope
Kennan, Ruth M
Rood, Julian I
Katz, Margaret Ethel
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
Bentham Open
Place of publication
Netherlands
DOI
10.2174/1874285800802010001
UNE publication id
une:3975
Abstract
The Gram-negative anaerobic pathogen 'Dichelobacter nodosus' carries several genetic elements that integrate into the chromosome. These include the 'intA', 'intB', 'intC' and 'intD' elements, which integrate adjacent to 'csrA' and 'pnpA', two putative global regulators of virulence and the virulence-related locus, 'vrl', which integrates into 'ssrA'. Treatment of 'D. nodosus' strains with ultraviolet light resulted in the isolation of DinoHI, a member of the 'Siphoviridae' and the first bacteriophage to be identified in 'D. nodosus'. Part of the DinoHI genome containing the packaging site is found in all 'D. nodosus' strains tested and is located at the end of the 'vrl', suggesting a role for DinoHI in the transfer of the 'vrl' by transduction. Like the intB element, the DinoHI genome contains a copy of 'regA' which has similarity to the repressors of lambdoid bacteriophages, suggesting that the maintenance of DinoHI and the intB element may be co-ordinately controlled.
Link
Citation
The Open Microbiology Journal, v.2, p. 1-9
ISSN
1874-2858
Start page
1
End page
9

Files:

NameSizeformatDescriptionLink