Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/3876
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dc.contributor.authorIslam, Afm Fakhrulen
dc.contributor.authorYoung, Pen
dc.contributor.authorWalkden-Brown, Steve Williamen
dc.contributor.authorBurgess, Susanen
dc.contributor.authorGroves, Peter Johnen
dc.date.accessioned2009-12-17T12:09:00Z
dc.date.issued2001en
dc.identifier.citationAvian Pathology, 30(6), p. 621-628en
dc.identifier.issn1465-3338en
dc.identifier.issn0307-9457en
dc.identifier.urihttps://hdl.handle.net/1959.11/3876en
dc.description.abstractThe polymerase chain reaction (PCR) has recently emerged as an additional tool for the monitoring and diagnosis of Marek's disease. We investigated a number of factors that may influence the interpretation of PCR results in commercial broiler chickens including the effects of route of infection and herpesvirus of turkeys (HVT)-vaccination status. We also investigated the suitability of peripheral blood lymphocytes (PBL) and spleen as tissues for Marek's disease virus (MDV) detection. HVT-vaccinated and unvaccinated commercial broiler chickens were challenged or not challenged with virulent MDV either by intraperitoneal injection or inhalation of feather dust containing the virus. Blood and spleen samples were collected at weekly intervals to day 35 post-infection for PCR of spleen or PBL. Live weight and lymphoid organ weights were also measured. Spleen and PBL were found to provide similar sensitivity of detection of MDV with a small advantage in favour of spleen. In terms of the timing of detection of MDV, intraperitoneal challenge broadly mimicked natural challenge via inhalation, although infection of birds by inhalation of infective feather dust resulted in slightly later but more complete detection of MDV in challenged birds. Vaccination with HVT delayed the detection of MDV by approximately 10 to 14 days and did not protect against the reduced growth observed in challenged chickens at day 35 post-challenge.en
dc.languageenen
dc.publisherTaylor & Francis Ltden
dc.relation.ispartofAvian Pathologyen
dc.titleMarek's disease in broiler chickens: effect of route of infection and herpesvirus of turkey-vaccination status on detection of virus from blood or spleen by polymerase chain reaction, and on weights of birds, bursa and spleenen
dc.typeJournal Articleen
dc.identifier.doi10.1080/03079450120092116en
dc.subject.keywordsVeterinary Parasitologyen
local.contributor.firstnameAfm Fakhrulen
local.contributor.firstnamePen
local.contributor.firstnameSteve Williamen
local.contributor.firstnameSusanen
local.contributor.firstnamePeter Johnen
local.subject.for2008070708 Veterinary Parasitologyen
local.subject.seo2008960403 Control of Animal Pests, Diseases and Exotic Species in Farmland, Arable Cropland and Permanent Cropland Environmentsen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.schoolSchool of Environmental and Rural Scienceen
local.profile.emailswalkden@une.edu.auen
local.profile.emailsburgess@une.edu.auen
local.profile.emailpgroves2@une.edu.auen
local.output.categoryC1en
local.record.placeauen
local.record.institutionUniversity of New Englanden
local.identifier.epublicationsrecordpes:5113en
local.publisher.placeLondon, United Kingdomen
local.format.startpage621en
local.format.endpage628en
local.peerreviewedYesen
local.identifier.volume30en
local.identifier.issue6en
local.title.subtitleeffect of route of infection and herpesvirus of turkey-vaccination status on detection of virus from blood or spleen by polymerase chain reaction, and on weights of birds, bursa and spleenen
local.contributor.lastnameIslamen
local.contributor.lastnameYoungen
local.contributor.lastnameWalkden-Brownen
local.contributor.lastnameBurgessen
local.contributor.lastnameGrovesen
dc.identifier.staffune-id:swalkdenen
dc.identifier.staffune-id:sburgessen
dc.identifier.staffune-id:pgroves2en
local.profile.orcid0000-0002-0638-5533en
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.profile.roleauthoren
local.identifier.unepublicationidune:3971en
dc.identifier.academiclevelAcademicen
dc.identifier.academiclevelAcademicen
local.title.maintitleMarek's disease in broiler chickensen
local.output.categorydescriptionC1 Refereed Article in a Scholarly Journalen
local.description.statisticsepubsVisitors: 236<br />Views: 235<br />Downloads: 0en
local.search.authorIslam, Afm Fakhrulen
local.search.authorYoung, Pen
local.search.authorWalkden-Brown, Steve Williamen
local.search.authorBurgess, Susanen
local.search.authorGroves, Peter Johnen
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