Please use this identifier to cite or link to this item: https://hdl.handle.net/1959.11/3876
Title: Marek's disease in broiler chickens: effect of route of infection and herpesvirus of turkey-vaccination status on detection of virus from blood or spleen by polymerase chain reaction, and on weights of birds, bursa and spleen
Contributor(s): Islam, Afm Fakhrul (author); Young, P (author); Walkden-Brown, Steve William (author)orcid ; Burgess, Susan (author); Groves, Peter John (author)
Publication Date: 2001
DOI: 10.1080/03079450120092116
Handle Link: https://hdl.handle.net/1959.11/3876
Abstract: The polymerase chain reaction (PCR) has recently emerged as an additional tool for the monitoring and diagnosis of Marek's disease. We investigated a number of factors that may influence the interpretation of PCR results in commercial broiler chickens including the effects of route of infection and herpesvirus of turkeys (HVT)-vaccination status. We also investigated the suitability of peripheral blood lymphocytes (PBL) and spleen as tissues for Marek's disease virus (MDV) detection. HVT-vaccinated and unvaccinated commercial broiler chickens were challenged or not challenged with virulent MDV either by intraperitoneal injection or inhalation of feather dust containing the virus. Blood and spleen samples were collected at weekly intervals to day 35 post-infection for PCR of spleen or PBL. Live weight and lymphoid organ weights were also measured. Spleen and PBL were found to provide similar sensitivity of detection of MDV with a small advantage in favour of spleen. In terms of the timing of detection of MDV, intraperitoneal challenge broadly mimicked natural challenge via inhalation, although infection of birds by inhalation of infective feather dust resulted in slightly later but more complete detection of MDV in challenged birds. Vaccination with HVT delayed the detection of MDV by approximately 10 to 14 days and did not protect against the reduced growth observed in challenged chickens at day 35 post-challenge.
Publication Type: Journal Article
Source of Publication: Avian Pathology, 30(6), p. 621-628
Publisher: Taylor & Francis Ltd
Place of Publication: London, United Kingdom
ISSN: 1465-3338
0307-9457
Field of Research (FOR): 070708 Veterinary Parasitology
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Statistics to Oct 2018: Visitors: 236
Views: 235
Downloads: 0
Appears in Collections:Journal Article

Files in This Item:
2 files
File Description SizeFormat 
Show full item record

SCOPUSTM   
Citations

12
checked on Nov 30, 2018

Page view(s)

54
checked on Feb 8, 2019
Google Media

Google ScholarTM

Check

Altmetric

SCOPUSTM   
Citations

 

WEB OF SCIENCETM
Citations

 

Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.