Rapid Communication: mapping of the Ca²⁺ 'ATPase of fast twitch 1 skeletal muscle sarcoplasmic reticulum' (ATP2A1) gene to porcine chromosome 3

Title
Rapid Communication: mapping of the Ca²⁺ 'ATPase of fast twitch 1 skeletal muscle sarcoplasmic reticulum' (ATP2A1) gene to porcine chromosome 3
Publication Date
2002
Author(s)
Ciobanu, D C
Zhang, Yuandan
( author )
OrcID: https://orcid.org/0000-0002-1998-3313
Email: yzhang4@une.edu.au
UNE Id une-id:yzhang4
Rothschild, Max F
Type of document
Journal Article
Language
en
Entity Type
Publication
Publisher
American Society of Animal Science
Place of publication
United States of America
UNE publication id
une:3715
Abstract
Locus Name: Ca²⁺ ATPase of fast twitch 1 skeletal muscle sarcoplasmic reticulum. Species: Sus scrofa. Locus Symbol: AMAL Source of Primers: Two sets of PCR primers were designed based on human ATP2A1 sequences (GenBank accession numbers U96773 and U96777) available in the GenBank database. Using pig genomic DNA as a template we used set A primers to amplify approximately 1,100 bp spanning exon 1 to exon 2 and set B primers to amplify 1,200 bp gene segment covering some of exon 8 through part of exon 9. These sequences were deposited in GenBank, accession number AY027797-AY027799. Primer Sequences: Primers derived from the porcine sequences were as follows: Set A: forward, 5'-GTC TCA GCC AGC CAA TCC CT-3' and reverse, 5'-AAG GAA ATG CAT GCG GCC AG-3'; Set B: forward, 5'-GGG CAC CAA CAT TGC AGC C-3' and reverse, 5'-CTT GCA GAC AGA CAT CTG GTT-3'.
Link
Citation
Journal of Animal Science, 80(5), p. 1386-1387
ISSN
1525-3163
0021-8812
Start page
1386
End page
1387

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