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|Title:||Rapid Communication: mapping of the Ca²⁺ 'ATPase of fast twitch 1 skeletal muscle sarcoplasmic reticulum' (ATP2A1) gene to porcine chromosome 3||Contributor(s):||Ciobanu, D C (author); Zhang, Yuandan (author); Rothschild, Max F (author)||Publication Date:||2002||Handle Link:||https://hdl.handle.net/1959.11/3625||Abstract:||Locus Name: Ca²⁺ ATPase of fast twitch 1 skeletal muscle sarcoplasmic reticulum. Species: Sus scrofa. Locus Symbol: AMAL Source of Primers: Two sets of PCR primers were designed based on human ATP2A1 sequences (GenBank accession numbers U96773 and U96777) available in the GenBank database. Using pig genomic DNA as a template we used set A primers to amplify approximately 1,100 bp spanning exon 1 to exon 2 and set B primers to amplify 1,200 bp gene segment covering some of exon 8 through part of exon 9. These sequences were deposited in GenBank, accession number AY027797-AY027799. Primer Sequences: Primers derived from the porcine sequences were as follows: Set A: forward, 5'-GTC TCA GCC AGC CAA TCC CT-3' and reverse, 5'-AAG GAA ATG CAT GCG GCC AG-3'; Set B: forward, 5'-GGG CAC CAA CAT TGC AGC C-3' and reverse, 5'-CTT GCA GAC AGA CAT CTG GTT-3'.||Publication Type:||Journal Article||Source of Publication:||Journal of Animal Science, 80(5), p. 1386-1387||Publisher:||American Society of Animal Science||Place of Publication:||Savoy, IL, USA||ISSN:||0021-8812
|Field of Research (FOR):||070201 Animal Breeding||Socio-Economic Outcome Codes:||830308 Pigs||Peer Reviewed:||Yes||HERDC Category Description:||C1 Refereed Article in a Scholarly Journal||Other Links:||http://jas.fass.org/cgi/reprint/80/5/1386?ck=nck||Statistics to Oct 2018:||Visitors: 248
|Appears in Collections:||Animal Genetics and Breeding Unit (AGBU)|
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