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Title: Rapid Communication: mapping of the Ca²⁺ 'ATPase of fast twitch 1 skeletal muscle sarcoplasmic reticulum' (ATP2A1) gene to porcine chromosome 3
Contributor(s): Ciobanu, D C (author); Zhang, Yuandan  (author); Rothschild, Max F (author)
Publication Date: 2002
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Abstract: Locus Name: Ca²⁺ ATPase of fast twitch 1 skeletal muscle sarcoplasmic reticulum. Species: Sus scrofa. Locus Symbol: AMAL Source of Primers: Two sets of PCR primers were designed based on human ATP2A1 sequences (GenBank accession numbers U96773 and U96777) available in the GenBank database. Using pig genomic DNA as a template we used set A primers to amplify approximately 1,100 bp spanning exon 1 to exon 2 and set B primers to amplify 1,200 bp gene segment covering some of exon 8 through part of exon 9. These sequences were deposited in GenBank, accession number AY027797-AY027799. Primer Sequences: Primers derived from the porcine sequences were as follows: Set A: forward, 5'-GTC TCA GCC AGC CAA TCC CT-3' and reverse, 5'-AAG GAA ATG CAT GCG GCC AG-3'; Set B: forward, 5'-GGG CAC CAA CAT TGC AGC C-3' and reverse, 5'-CTT GCA GAC AGA CAT CTG GTT-3'.
Publication Type: Journal Article
Source of Publication: Journal of Animal Science, 80(5), p. 1386-1387
Publisher: American Society of Animal Science
Place of Publication: Savoy, IL, USA
ISSN: 0021-8812
Field of Research (FOR): 070201 Animal Breeding
Socio-Economic Outcome Codes: 830308 Pigs
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
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Appears in Collections:Animal Genetics and Breeding Unit (AGBU)
Journal Article

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