Hooper, Stuart B; Kitchen, Marcus J; Irvine, Sarah C; Pavlov, Konstantin M; Lewis, Robert A; Wallace, Megan J; Yagi, Naoto; Uesugi, Kentaro; Morgan, Michael J; Hall, Chris; Siu, Karen K W; Williams, Ivan M; Siew, Melissa

Author(s)

Hooper, Stuart B

Kitchen, Marcus J

Irvine, Sarah C

Pavlov, Konstantin M

Lewis, Robert A

Wallace, Megan J

Yagi, Naoto

Uesugi, Kentaro

Morgan, Michael J

Hall, Chris

Siu, Karen K W

Williams, Ivan M

Siew, Melissa

Publication Date

2007

Abstract

Aeration of the lung and the transition to air-breathing at birth is fundamental to mammalian life and initiates major changes in cardiopulmonary physiology. However, the dynamics of this process and the factors involved are largely unknown, because it has not been possible to observe or measure lung aeration on a breath-by-breath basis. We have used the high contrast and spatial resolution of phase contrast X-ray imaging to study lung aeration at birth in spontaneously breathing neonatal rabbits. As the liquid-filled fetal lungs provide little absorption or phase contrast, they are not visible and only become visible as they aerate, allowing a detailed examination of this process. Pups were imaged live from birth to determine the timing and spatial pattern of lung aeration, and relative levels of lung aeration were measured from the images using a power spectral analysis. We report the first detailed observations and measurements of lung aeration, demonstrating its dependence on inspiratory activity and body position; dependent regions aerated at much slower rates. The air/liquid interface moved toward the distal airways only during inspiration, with little proximal movement during expiration, indicating that transpulmonary pressures play an important role in airway liquid clearance at birth. Using these imaging techniques, the dynamics of lung aeration and the critical role it plays in regulating the physiological changes at birth can be fully explored.—Hooper, S. B., Kitchen, M. J., Wallace, M. J., Yagi, N., Uesugi, K., Morgan, M. J., Hall, C., Siu, K. K. W., Williams, I. M., Siew, M., Irvine, S. C., Pavlov, K., Lewis, R. A. Imaging lung aeration and lung liquid clearance at birth. FASEB J. 21, 3329–3337 (2007)

Citation

FASEB Journal, 21(12), p. 3329-3337

ISSN

1530-6860

0892-6638

Link

https://hdl.handle.net/1959.11/3616

Publisher

Federation of American Societies for Experimental Biology

Title

Imaging lung aeration and lung liquid clearance at birth

Type of document

Journal Article

Entity Type

Publication

Author(s)	Hooper, Stuart B Kitchen, Marcus J Irvine, Sarah C Pavlov, Konstantin M Lewis, Robert A Wallace, Megan J Yagi, Naoto Uesugi, Kentaro Morgan, Michael J Hall, Chris Siu, Karen K W Williams, Ivan M Siew, Melissa
Publication Date	2007
Abstract	Aeration of the lung and the transition to air-breathing at birth is fundamental to mammalian life and initiates major changes in cardiopulmonary physiology. However, the dynamics of this process and the factors involved are largely unknown, because it has not been possible to observe or measure lung aeration on a breath-by-breath basis. We have used the high contrast and spatial resolution of phase contrast X-ray imaging to study lung aeration at birth in spontaneously breathing neonatal rabbits. As the liquid-filled fetal lungs provide little absorption or phase contrast, they are not visible and only become visible as they aerate, allowing a detailed examination of this process. Pups were imaged live from birth to determine the timing and spatial pattern of lung aeration, and relative levels of lung aeration were measured from the images using a power spectral analysis. We report the first detailed observations and measurements of lung aeration, demonstrating its dependence on inspiratory activity and body position; dependent regions aerated at much slower rates. The air/liquid interface moved toward the distal airways only during inspiration, with little proximal movement during expiration, indicating that transpulmonary pressures play an important role in airway liquid clearance at birth. Using these imaging techniques, the dynamics of lung aeration and the critical role it plays in regulating the physiological changes at birth can be fully explored.—Hooper, S. B., Kitchen, M. J., Wallace, M. J., Yagi, N., Uesugi, K., Morgan, M. J., Hall, C., Siu, K. K. W., Williams, I. M., Siew, M., Irvine, S. C., Pavlov, K., Lewis, R. A. Imaging lung aeration and lung liquid clearance at birth. FASEB J. 21, 3329–3337 (2007)
Citation	FASEB Journal, 21(12), p. 3329-3337
ISSN	1530-6860 0892-6638
Link	https://hdl.handle.net/1959.11/3616
Publisher	Federation of American Societies for Experimental Biology
Title	Imaging lung aeration and lung liquid clearance at birth
Type of document	Journal Article
Entity Type	Publication

Imaging lung aeration and lung liquid clearance at birth

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