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https://hdl.handle.net/1959.11/3398
Title: | Ontogeny of Leptin and its Receptor Expression in Mouse Testis During the Postnatal Period | Contributor(s): | Herrid, Muren (author); O'Shea, Timothy (author); McFarlane, James R (author) | Publication Date: | 2008 | DOI: | 10.1002/mrd.20796 | Handle Link: | https://hdl.handle.net/1959.11/3398 | Abstract: | The mechanism by which leptin regulate male reproductive development during postnatal periods remain to be determined. Using immunohistochemistry and reverse transcription-polymerase chain reaction (RT-PCR), we established that leptin is expressed in mouse testis with a cell-type and stage-dependent manner during the postnatal period. In testes of 5-day-old mice, leptin expression was mainly restricted to gonocytes, whereas the immunostaining of leptin was confined to spermatogonia in 10-day-old testes. From Day 20 and onwards, stage-specific expression of leptin was evident in spermatocytes at stages VII-XII of the cycle in the seminiferous epithelium. RT-PCR showed that leptin and its receptor isoforms, Ob-Ra, Ob-Rb, and Ob-Re were all expressed in testes from 5- to 60-day-old mice. The mRNA for Ob-Ra and Ob-Re, but not Ob-Rb or leptin were identified in both immature (14-day-old) and adult (60-day-old) isolated Leydig cells. These results suggest that besides its primary actions at the hypothalamic-pituitary level, leptin has direct effects in the proliferation, differentiation of germ cells and the modulation of testicular steroidogenesis, using both autocrine and paracrine mechanisms. | Publication Type: | Journal Article | Source of Publication: | Molecular Reproduction and Development, 75(5), p. 874-880 | Publisher: | John Wiley & Sons, Inc | Place of Publication: | United States of America | ISSN: | 1098-2795 1040-452X |
Fields of Research (FoR) 2008: | 110306 Endocrinology 111404 Reproduction |
Socio-Economic Objective (SEO) 2008: | 920114 Reproductive System and Disorders | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
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Appears in Collections: | Journal Article |
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