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Rapid communication: physical and linkage mapping of the porcine connexin 37 (CX37) gene |
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American Society of Animal Science |
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Source and Description of Primers: A set of primers (F1, R1) was designed from the porcine CX37 mRNA sequence (GenBank Accession no. X86024) as well as an additional reverse primer (R2) from human (GenBank Accession no. 6093424) and murine (GenBank Accesion no. NM_008120) CX37 consensus sequence. The F1 and R2 primers were used to amplify porcine CX37 from genomic DNA. Using sequence obtained from the amplified product, an additional pig-specific forward primer (F2) was designed. Primer Sequences: F1: 5'-TTC CTG GAG AAG CTG CTG GA-3'; R1: 5'-CGA GAT CTT GGC CAT CTG TC- 3'; F2: 5'-ACT CGA CCG TGG TGG GCA A-3'; R2: 5'- GTG GTC AGG TTG GCC CAG TT-3'. |
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Journal of Animal Science, 80(5), p. 1375-1376 |
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