Tuggle, Christopher K

Green, Jon A

Tack, Dylan

Robinson, Natalie

O'Leary, Brian

Scheetz, Todd

Casavant, Thomas

Pomp, Daniel

Edeal, J Brad

Zhang, Yuandan

Rothschild, Max F

Garwood, Kevin

Fitzsimmons, Carolyn

Beavis, William

Woods, Rami

Prather, Randall S

Malchenko, Sergei

Soares, Bento

Kucaba, Tamara

Crouch, Keith

Smith, Christina

2003

A molecular understanding of porcine reproduction is of biological interest and economic importance. Our Midwest Consortium has produced cDNA libraries containing the majority of genes expressed in major female reproductive tissues, and we have deposited into public databases 21,499 expressed sequence tag (EST) gene sequences from the 3¢ end of clones from these libraries. These sequences represent 10,574 different genes, based on sequence comparison among these data, and comparison with existing porcine ESTs and genes indicate as many as 4652 of these EST clusters are novel. In silico analysis identified sequences that are expressed in specific pig tissues or organs and confirmed the broad expression in pig for many genes ubiquitously expressed in human tissues. Furthermore, we have developed computer software to identify sequence similarity of these pig genes with their human counterparts, and to extract the mapping information of these human homologues from genome databases. We demonstrate the utility of this software for comparative mapping by localizing 61 genes on the porcine physical map for Chromosomes (Chrs) 5, 10, and 14.

Mammalian Genome, 14(14), p. 565-579

1432-1777

0938-8990

https://hdl.handle.net/1959.11/3249

en

Springer New York LLC

EST-based gene discovery in pig: virtual expression patterns and comparative mapping to human

Journal Article

Publication