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Title: EST-based gene discovery in pig: virtual expression patterns and comparative mapping to human
Contributor(s): Tuggle, Christopher K (author); Green, Jon A (author); Tack, Dylan (author); Robinson, Natalie (author); O'Leary, Brian (author); Scheetz, Todd (author); Casavant, Thomas (author); Pomp, Daniel (author); Edeal, J Brad (author); Zhang, Yuandan  (author)orcid ; Rothschild, Max F (author); Garwood, Kevin (author); Fitzsimmons, Carolyn (author); Beavis, William (author); Woods, Rami (author); Prather, Randall S (author); Malchenko, Sergei (author); Soares, Bento (author); Kucaba, Tamara (author); Crouch, Keith (author); Smith, Christina (author)
Publication Date: 2003
DOI: 10.1007/s00335-002-2263-7
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Abstract: A molecular understanding of porcine reproduction is of biological interest and economic importance. Our Midwest Consortium has produced cDNA libraries containing the majority of genes expressed in major female reproductive tissues, and we have deposited into public databases 21,499 expressed sequence tag (EST) gene sequences from the 3¢ end of clones from these libraries. These sequences represent 10,574 different genes, based on sequence comparison among these data, and comparison with existing porcine ESTs and genes indicate as many as 4652 of these EST clusters are novel. In silico analysis identified sequences that are expressed in specific pig tissues or organs and confirmed the broad expression in pig for many genes ubiquitously expressed in human tissues. Furthermore, we have developed computer software to identify sequence similarity of these pig genes with their human counterparts, and to extract the mapping information of these human homologues from genome databases. We demonstrate the utility of this software for comparative mapping by localizing 61 genes on the porcine physical map for Chromosomes (Chrs) 5, 10, and 14.
Publication Type: Journal Article
Source of Publication: Mammalian Genome, 14(14), p. 565-579
Publisher: Springer New York LLC
Place of Publication: United States of America
ISSN: 1432-1777
Fields of Research (FoR) 2008: 070201 Animal Breeding
Socio-Economic Objective (SEO) 2008: 830308 Pigs
Peer Reviewed: Yes
HERDC Category Description: C1 Refereed Article in a Scholarly Journal
Appears in Collections:Animal Genetics and Breeding Unit (AGBU)
Journal Article

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