Please use this identifier to cite or link to this item:
https://hdl.handle.net/1959.11/31397
Title: | Morphological and molecular variability in some Iranian almond genotypes and related Prunus species and their potentials for rootstock breeding | Contributor(s): | Nikoumanesh, Kianoush (author) ; Ebadi, Ali (author); Zeinalabedini, Mehrshad (author); Gogorcenac, Yolanda (author) | Publication Date: | 2011-05-25 | Early Online Version: | 2011-04-02 | DOI: | 10.1016/j.scienta.2011.03.017 | Handle Link: | https://hdl.handle.net/1959.11/31397 | Abstract: | In this study, in order to know the variability for a rootstock breeding program genetic diversity and relationships among 55 Iranian almond genotypes and seven related Prunus species were investigated. Morphological and molecular analyses were used. Principal component analysis showed that three components explained 67.6% of the total morphological variation for the first year and 68.06% for the second year of the study. Leaf traits were predominant in the first component and contributed most of the total variation. Leaf length and width, as well as, leaf area were highly correlated with each other and correlated to vigor. Also a negative correlation was found between leaf length/width ratio and vigor. Ward's method was used to construct cluster from morphological data which allocated individuals into their respective species. Out of 100 pre-screened RAPD primers, 16 with reproducible bands and maximum polymorphism were selected. Two-hundred and sixty bands were scored of which 250 of them were polymorphic. Average value of polymorphism per primer was 95.81% and maximum value for polymorphism (100%) was obtained from TIBMBA-14, TIBMBA-17, TIBMBB-05, TIBMBB-08, TIBMBD-09, and TIBMBD-10. On the other hand, the minimum value was obtained from TIBMBB-16 (86%). Primer TIBMBB-5 gave the maximum number of bands (25 fragments) and the minimum obtained from TIBMBE-18 (11 fragments). Genetic similarity based on Jaccard's coefficient ranged from 0.28 to 0.79 with an average of 0.53. Molecular analysis revealed a high degree of separation among samples regarding their geographical origin. Correlation between two approaches was low (R =−0.38). High molecular and morphological variability indicated that this collection includes rich and valuable plant materials for almond rootstock breeding. | Publication Type: | Journal Article | Source of Publication: | Scientia Horticulturae, 129(1), p. 108-118 | Publisher: | Elsevier BV | Place of Publication: | Netherlands | ISSN: | 0304-4238 | Fields of Research (FoR) 2020: | 300803 Horticultural crop improvement (incl. selection and breeding) | Socio-Economic Objective (SEO) 2020: | 260513 Stone fruit (excl. avocado) | Peer Reviewed: | Yes | HERDC Category Description: | C1 Refereed Article in a Scholarly Journal |
---|---|
Appears in Collections: | Animal Genetics and Breeding Unit (AGBU) Journal Article |
Files in This Item:
File | Size | Format |
---|
SCOPUSTM
Citations
42
checked on Nov 18, 2023
Page view(s)
1,318
checked on Nov 19, 2023
Items in Research UNE are protected by copyright, with all rights reserved, unless otherwise indicated.